| 麋鹿产气荚膜梭菌分离鉴定方法的建立与初步应用 |
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| 引用本文： | 邱慧玲,陈甫,费荣梅,唐名艳.麋鹿产气荚膜梭菌分离鉴定方法的建立与初步应用[J].畜牧与兽医,2011,43(10). |
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| 作者姓名： | 邱慧玲 陈甫 费荣梅 唐名艳 |
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| 作者单位： | 1. 南京农业大学动物医学院,江苏南京,210095
2. 青岛农业大学动物科技学院,山东青岛,266109 |
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| 基金项目： | 野生动物疫病监测和预警系统维护-野生动物疫源疫病本底调查项目资助 |
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| 摘 要： | 探索了产气荚膜梭菌在营养琼脂、甘露醇卵黄琼脂和血琼脂平板上的菌落形态和培养特点,优化了产气荚膜梭菌的分离方法,并对分离菌株进行生化鉴定;在此基础上对北京南海子麋鹿苑和江苏大丰麋鹿国家级自然保护区的麋鹿自然感染情况进行了调查。结果发现,粪样中的产气荚膜梭菌在营养琼脂上生长呈半透明边缘不整的白色菌落,接种甘露醇卵黄琼脂和血琼脂平板,分别出现伴有卵磷脂酶乳光浑浊带的粉红色火山口状菌落和伴有双溶血环的灰绿色勋章样菌落。生化试验结果确认这些分离株均为产气荚膜梭菌。对北京南海子麋鹿苑和江苏大丰麋鹿国家级自然保护区麋鹿粪样检测发现,阳性率分别为13.04%(3/23)和19.51%(8/41)。说明本研究建立的麋鹿产气荚膜梭菌分离鉴定方法简便快速,确实可行;麋鹿产气荚膜梭菌自然感染率较高,应加强麋鹿产气荚膜梭菌病的防控。
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| 关 键 词： | 麋鹿 产气荚膜梭菌 分离 鉴定 |
Establishment of the isolation and identification method for Clostridium perfringens in Elaphurus davidianus and its preliminary application |
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| QIU Hui-ling,CHEN Fu,FEI Rong-mei,TANG Ming-yan.Establishment of the isolation and identification method for Clostridium perfringens in Elaphurus davidianus and its preliminary application[J].Animal Husbandry & Veterinary Medicine,2011,43(10). |
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| Authors: | QIU Hui-ling CHEN Fu FEI Rong-mei TANG Ming-yan |
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| Institution: | QIU Hui-ling1,CHEN Fu2,FEI Rong-mei1,TANG Ming-yan1(1.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China,2.College of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China) |
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| Abstract: | To establish a simple and rapid method for isolating and identifying Clostridium perfringens in the feces of Elaphurus davidianus, the colony and culture characteristics of C.perfringens were studied on the nutrient agar,mannitol egg yolk agar,and blood agar media.And then the C.perfringens infections in Elaphurus davidianus in Beijing Nanhaizi Elaphurus davidianus Park and Jiangsu Dafeng Elaphurus davidianus National Nature Reserve were investigated.C.perfringens colonies showed white translucent mycelium ... |
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| Keywords: | Elaphurus davidianus Clostridium perfringens isolation identification |
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