| 温氏支原体感染动物模型的建立 |
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| 引用本文： | 王鹏,赵彦岭,张富梅.温氏支原体感染动物模型的建立[J].畜牧与兽医,2011,43(8). |
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| 作者姓名： | 王鹏 赵彦岭 张富梅 |
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| 作者单位： | 1. 河北北方学院动物科技学院,河北张家口,075000
2. 河北省兽药监察所,河北石家庄,050051 |
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| 基金项目： | 河北省畜牧局课题(2006-11) |
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| 摘 要： | 为促进温氏支原体的深入研究,本试验建立了温氏支原体感染动物模型。选择6～8周龄健康雄性昆明种小鼠60只,随机分为A、B、C、D 4组,试验组(A、B、C)分别以不同方式感染温氏支原体,D组为对照组。结果表明,腹腔注射一定剂量高感染强度的牛红细胞悬液,同时注射免疫抑制剂地塞米松组(C组)表现为首现期出现早,且高峰期红细胞感染率高。应用悬滴镜检法和PCR诊断方法对感染小鼠血样进行鉴定,均符合温氏支原体特征。选择对照组小鼠和感染率高、临床症状较为明显的C组小鼠各10只,进行血液生理生化指标测定(RBC、WBC、Hb、TP、G、ALT、AST),结果呈现出与牛感染温氏支原体相同的规律。
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| 关 键 词： | 小白鼠 温氏支原体 动物模型 |
Establishment of animal models for Eperythrozoon wenyoni infection |
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| WANG Peng,ZHAO Yan-ling,ZHANG Fu-mei.Establishment of animal models for Eperythrozoon wenyoni infection[J].Animal Husbandry & Veterinary Medicine,2011,43(8). |
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| Authors: | WANG Peng ZHAO Yan-ling ZHANG Fu-mei |
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| Institution: | WANG Peng1,ZHAO Yan-ling2,ZHANG Fu-mei1(1.The College of Animal Science & Technology,Hebei North University,Zhangjiakou 075000,China,2.Hebei Animal Remedy Supervision Offfice,Shijiazhuang 050051,China) |
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| Abstract: | This study is to establish an animal model for Eperythrozoon wenyoni infection.Sixty Kunming mice of 6 to 8 weeks old were selected,and divided into four groups randomly: A,B,C and D groups.The experimental groups(A,B and C) were infected with E.wenyoni at the different ways.The results showed that the appearance period was earlier,and the infective rate of erythrocyte(RBC) was higher among peak period in group C,in which bovine RBC suspension with higher infective intensity was injected and the immunosuppr... |
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| Keywords: | mice E wenyoni animal model |
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