| 牛皮蝇蛆病间接ELISA诊断方法的建立 |
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| 引用本文： | 王文龙,阮风雷,刘春霞,初一鑫,呼和巴特尔.牛皮蝇蛆病间接ELISA诊断方法的建立[J].畜牧与兽医,2011,43(10). |
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| 作者姓名： | 王文龙 阮风雷 刘春霞 初一鑫 呼和巴特尔 |
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| 作者单位： | 1. 内蒙古农业大学兽医学院,内蒙古呼和浩特,010018
2. 内蒙古农业大学生命科学学院,内蒙古呼和浩特,010018 |
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| 基金项目： | 教育部“春晖计划”合作项目(Z2007-1-01018); 国家自然科学基金(30360082) |
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| 摘 要： | 以纹皮蝇Ⅰ期幼虫粗提蛋白为包被抗原,通过方阵试验确定了血清最佳稀释倍数为80倍,抗原最佳包被浓度为26.64μg/mL,并对其特异性、敏感性和重复性进行了试验,建立了牛皮蝇蛆病间接ELSIA诊断方法。再以建立的ELISA诊断方法对采自内蒙古地区的233份牛血清进行了检测。结果表明,所建立的牛皮蝇蛆病间接ELISA诊断方法具有较好的特异性、敏感性和可重复性,可用于牛皮蝇蛆病的血清学检测。
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| 关 键 词： | 牛皮蝇蛆病 虫体抗原 间接ELISA |
Establishment of an indirect ELISA for the detection of hypodermiosis in cattle |
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| WANG Wen-long,RUAN Feng-lei,LIU Chun-xia,CHU Yi-xin,HUHE Bateer.Establishment of an indirect ELISA for the detection of hypodermiosis in cattle[J].Animal Husbandry & Veterinary Medicine,2011,43(10). |
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| Authors: | WANG Wen-long RUAN Feng-lei LIU Chun-xia CHU Yi-xin HUHE Bateer |
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| Institution: | WANG Wen-long1,RUAN Feng-lei1,LIU Chun-xia2,CHU Yi-xin1,HUHE Bateer1(1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Huhhot 010018,China,2.College of Life Science,China) |
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| Abstract: | An indirect ELISA for hypodermiosis was established with antigens prepared from the first instar larvae of Hypoderma lineatum.The ideal serum concentration of 80 dilutions and the antigen coating concentration of 26.64 μg/mL were obtained by square matrix experiment,and then the specificity,sensitivity and repeatability of the diagnostic method were tested.233 bovine sera from the Inner Mongolia region were tested by the indirect ELISA.The results demonstrated that the established indirect ELISA for hypodermiosis with higher specificity,sensitivity and repeatability,could be used for the serological investigation of hypodermiosis. |
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| Keywords: | hypodermiosis larvae antigen indirect ELISA |
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