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| 牛体外受精胚胎培养技术研究 | |
| 引用本文： | 桑国俊,余四九,贺奋义,郭海龙,邱忠玉,豆有院,王珂,丁春贤.牛体外受精胚胎培养技术研究[J].畜牧与兽医,2009,41(3). |
| 作者姓名： | 桑国俊 余四九 贺奋义 郭海龙 邱忠玉 豆有院 王珂 丁春贤 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃兰州730070;甘肃省畜牧兽医研究所,甘肃平凉744000 2. 甘肃农业大学动物医学院,甘肃兰州,730070 3. 甘肃省畜牧兽医研究所,甘肃平凉,744000 |
| 基金项目： | 甘肃省农业生物技术项目 |
| 摘 要： | 在COCs体外培养过程中添加共培养物质和采用不同体积的液滴进行培养,以探讨牛卵母细胞的体外培养效果。结果表明,卵丘细胞、卵丘细胞+bFF共培养系统对胚胎发育均有促进作用,其中以卵丘细胞+bFF共培养系统对桑椹胚和囊胚的发育最好,其囊胚率为10.63%,显著高于对照组的7.23%(P<0.05),高于卵丘细胞组的8.50%(P>0.05)。用培养皿微滴培养(50μL)和用4孔板大体积培养(500μL)的试验表明,大体积培养比微滴培养有优势,桑椹胚和囊胚率均较高,其中囊胚率为10.23%,显著高于培养皿微滴培养的9.07%(P<0.01)。 |
| 关 键 词： | 牛 卵母细胞 卵丘细胞 卵泡液 体外培养 |
Technological investigation on the bovine oocyte in vitro culture |
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| SANG Guo-jun,YU Si-jiu,HE Fen-yi,GUO Hai-long,QIU Zhong-yu,DOU You-yuan,WANG Ke,DING Chun-xian.Technological investigation on the bovine oocyte in vitro culture[J].Animal Husbandry & Veterinary Medicine,2009,41(3). | |
| Authors: | SANG Guo-jun YU Si-jiu HE Fen-yi GUO Hai-long QIU Zhong-yu DOU You-yuan WANG Ke DING Chun-xian |
| Abstract: | In order to develop an efficient culture system of COCs in vitro,two methods which one was co-culture system with cumulus cell and/or bFF,and another was different volume of culture medium with 50 and/or 500 μL were investigated.The results indicated that the blastocyst rate of co-culture system with cumulus cell+bFF were significantly higher than the cumulus cell and control groups(P<0.05).In addition,the blastocyst rate of culture medium volume of 500 μL were significantly higher than that of 50 μL(P<0.01).Together,the co-culture system with cumulus cell and/or bFF and culture medium volume of 500 μL could improve the embryo development in vitro culture. |
| Keywords: | bovine oocyte cumulus cell bFF IVC |
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