L-肉碱对猪精液冷冻保存效果的研究

本试验用5%二甲基甲酰胺(DMF)替代甘油作为冷冻保护剂,研究不同浓度(0、0.02、0.04、0.06、0.08 mg/mL)L-肉碱对猪精液冻后常规指标(精子活率、线粒体活性、顶体完整性、质膜完整性)、过氧化氢酶(CAT)和总抗氧化酶(T-AOC)活性以及丙二醛(MDA)含量的影响。结果表明:添加0.04和0.06 mg/mL的L-肉碱可有效改善猪精液冷冻后效果。0.04 mg/mL组可显著提高精子冻后活率和线粒体活性(P<0.05);0.06 mg/mL组可显著提高顶体完整性和质膜完整性(P<0.05)。添加0.06 mg/mL L-肉碱可显著提高冷冻后精子内T-AOC酶活性并且抑制MDA的产生(P<0.05),但CAT活性与0.04 mg/mL组差异不显著。在冷冻稀释液中添加0.06 mg/mL L-肉碱可以提高猪精液冷冻保存效果。

Cryoprotective effects of L-carnitine on frozen-thawed semen in the pig

In this experiment,glycerol was replaced with 5% dimethyl formamide(DMF) completely and the L-carnitine of different concentrations(0、0.02、0.04、0.06、0.08 mg/mL) were added in the ordinary dilution,then the routine indicaters(sperm viability,mitochondrial activity,acrosome integrity and membrane integrity),the activity of CAT(Catalase) and T-AOC(total antioxidant capacity) and the content of MDA(Malondialdehyde) of frozen-thawed boar sperm were analyzed.The result showed that the cryoprotective effect were improved with addition of 0.04 and 0.06 mg/mL L-carnitine.When the L-carnitine concentration was 0.04 mg/mL,the sperm viability and mitochondrial activity were significantly higher than that of control group and other groups(P<0.05).When the L-carnitine concentration was 0.06 mg/mL,the acrosome integrity and membrane integrity were significantly higher than that of control group and other groups(P<0.05).The activity of T-AOC was increased and the content of MDA was decreased significantly with addition of 0.06 mg/mL L-carnitine,but there have no significant differences on the activity of CAT between 0.04 and 0.06 mg/mL groups.These results indicated that L-carnitine could improve boar semen quality after cryoprotection and the appropriate addition of L-carnitine in ordinary dilution was 0.06 mg/mL.