马鹿精子形态与超微结构研究

文中对马鹿(Cervus elaphus)精子形态与超微结构进行了研究。马鹿精子经固定、脱水、置换、包埋和聚合,用超薄切片机切片,再用醋酸双氧铀、柠檬酸铅染片,最后于透射电镜下观察其超微结构的变化。观察结果表明:马鹿精子全长(58.75±2.35)μm,由头部(8.93±0.24)μm、颈部(1.00±0.16)μm和尾部(48.18±1.18)μm三部分组成;头部呈扁卵圆状,绝大部分被浓缩且电子密度较高的精核所占据,顶体似帽状扣在精核之上,约占头部的2/3,其前部较为膨大;颈部位于头部和尾部之间,这个区域较短;尾部可分为中段、主段和终段。马鹿尾部轴丝的结构类型为"9+2";微管结构类型为"9+9+2"。

Morphology and Ultrastructure of Waptiti(Cervus elaphus) Sperm

The morphology and ultrastructure Wapiti(Cervus elaphus) sperm were studied.The wapiti sperm samples in vitro were treated with a series of procedure to get into slices,which needed to be dyed,the sperm ultrastructure were observed and the results showed that wapiti sperm was consisted of three parts : the head(58.75±2.35) μm long,the neck(8.93±0.24) μm long and the tail(48.18±1.18) μm long.The shape of the head was like a stick and most part of it was occupied by concentrated sperm nucleus with high-electronic density.The acrosome which was almost 2/3 of the head and had an expanded fore part was like a cap upon the sperm nucleus.Between the head and the tail there was the neck,and the length of the part was not long.The tail consisted of the middle,the principal and the end pieces.The axoneme structural type of wapiti sperm tail was "9+2";the microtubule structural type was "9+9+2".