| 珍珠鸡源IBDV的分离鉴定及其卵黄抗体的制备 |
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| 引用本文： | 徐亚萍,曾祥伟,李志军,李梦溪.珍珠鸡源IBDV的分离鉴定及其卵黄抗体的制备[J].经济动物学报,2012,16(2):76-78. |
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| 作者姓名： | 徐亚萍 曾祥伟 李志军 李梦溪 |
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| 作者单位： | 东北林业大学野生动物资源学院,哈尔滨,150040 |
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| 基金项目： | 东北林业大学国家级大学生创新性实验计划项目(1110225066);中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室开放基金项目(SKLVBF201201) |
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| 摘 要： | 从一群发病的珍珠鸡中分离到1株病毒,经鸡胚接种、琼脂扩散试验、PCR快速检测试验和病毒基因序列的测定,证实了所分离的病毒为IBDV。将该病毒制成灭活疫苗,接种开产的蛋鸡制备卵黄抗体。结果显示该卵黄抗体经免疫扩散试验测定抗体效价可达1∶256,符合无菌标准,对雏鸡也没有任何不良反应。临床试验表明:该卵黄抗体对该养殖场珍珠鸡的传染性法氏囊病的治疗有效率达91%、预防有效率达100%。
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| 关 键 词： | 珍珠鸡 鸡传染性法氏囊病病毒 分离鉴定 卵黄抗体 |
Isolation and Identification of Infectious Bursal Disease Virus from Guinea Fowl and Preparation of Egg Yolk Immunoglobulin |
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| XU Ya-ping,ZENG Xiang-wei,LI Zhi-jun,LI Meng-xi.Isolation and Identification of Infectious Bursal Disease Virus from Guinea Fowl and Preparation of Egg Yolk Immunoglobulin[J].Journal of Economic Animal,2012,16(2):76-78. |
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| Authors: | XU Ya-ping ZENG Xiang-wei LI Zhi-jun LI Meng-xi |
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| Institution: | (College of Wildlife Resources,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | A field virus isolated from guinea fowl was identified to be an infectious bursal disease virus(IBDV) by chicken embryo inoculation test,agar-gel diffusion test,PCR rapid detection test and virus gene sequencing.The egg laying chicken were immunized with inactivated oil emulsion vaccine developed with the infectious bursal disease virus isolated from guinea fowl to prepare egg yolk immunoglobulin.The result showed that the egg yolk immunoglobulin titer of IBDV determined by APG was 1∶256 and no bacteria were found in the egg yolk immunoglobulin and no any obvious adverse reaction to the chicken.The clinical trial showed that the effective rate of treatment was 91% and effective rate of prevention was 100% for the chicken. |
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| Keywords: | guinea fowl IBDV isolation and identification egg yolk immunoglobulin |
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