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| 驯鹿生长素Ghrelin cDNA的克隆 | |
| 引用本文： | 杨银凤,朱雪敏,余兴帮,赵艳芳.驯鹿生长素Ghrelin cDNA的克隆[J].经济动物学报,2007,11(4):196-198,210. |
| 作者姓名： | 杨银凤 朱雪敏 余兴帮 赵艳芳 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,呼和浩特,010018 2. 呼伦贝尔市兽医站,海拉尔,021000 3. 呼伦贝尔市鄂温克旗农牧业局,海拉尔,021000 |
| 基金项目： | 内蒙古农业大学博士启动基金资助项目(BJ04-2) |
| 摘 要： | 从驯鹿皱胃组织中提取总RNA,根据已发表的驯鹿生长素Ghrelin基因序列设计并合成引物,通过反转录-聚合酶链式反应(RT-PCR)进行cDNA扩增,获得了300 bp的片段,重组到pBlueselect T载体,经限制性内切酶谱分析和DNA序列测定分析,确认PCR产物为Ghrelin cDNA,为进一步研究Chrelin在驯鹿体内的分布及营养因素等对其基因表达的影响奠定基础。 |
| 关 键 词： | 生长素 驯鹿 cDNA |
| 文章编号： | 1007-7448（2007）04-0196-03 |
| 收稿时间： | 2007-05-31 |
| 修稿时间： | 2007年5月31日 |
cDNA Cloning of Reindeer Ghrelin |
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| YANG Yin-feng,ZHU Xue-min,YU Xing-bang,ZHAO Yan-fang.cDNA Cloning of Reindeer Ghrelin[J].Journal of Economic Animal,2007,11(4):196-198,210. | |
| Authors: | YANG Yin-feng ZHU Xue-min YU Xing-bang ZHAO Yan-fang |
| Abstract: | Ghrelin,an endogenous ligand for the growth hormone secretagogue receptor(GHS-R),is a kind of multi-peptide of 28 amino acid residues and can facilitate to release of GH.Total RNA was extracted from the abomasus tissue of reindeer and 300 bp gene fragment was obtained by RT-PCR with the primers which had been designed according to the published reindeer ghrelin gene sequence and synthesized.After being cloned into pBlueselect T vector,the PCR segment was determined as ghrelin cDNA by restriction endonuclease pattern analysis of recombinant plasmid and DNA sequencing. |
| Keywords: | ghrelin reindeer cDNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |