Isolation of Fasciola hepatica metacercariae by density gradients |
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Authors: | T R Fry J J Reddington R B Wescott R W Leid |
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Institution: | Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 U.S.A. |
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Abstract: | Fasciola hepatica metacercariae were purified in high yield, removing contaminating cyst walls and plant material by step gradients consisting of 10 ml of 60% Percoll (density = 1.08 g ml-1) and 10 ml of 50% Metrizamide (density = 1.25 g ml-1). Greater than 90% of the metacercariae applied to the density gradients were recovered. These isolated metacercariae had an in vitro excystment rate of greater than 80%, which was the same excystment rate as metacercariae not subjected to density gradient centrifugation. |
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Keywords: | Author to whom correspondence should be addressed |
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