| 维氏气单胞菌RCR快速检测方法的建立及初步应用 |
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| 引用本文： | 边 宇,单晓枫.维氏气单胞菌RCR快速检测方法的建立及初步应用[J].中国兽药杂志,2013,47(1):1-3. |
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| 作者姓名： | 边 宇 单晓枫 |
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| 作者单位： | 吉林农业大学动物科学技术学院,吉林农业大学动物科学技术学院 |
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| 基金项目： | 国家支撑计划项目（2010BAD04B01）；吉林省科技厅项目（20080218）；吉林农业大学青年启动基金（200904） |
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| 摘 要： | 以维氏气单胞菌的核酸酶基因为靶基因建立了PCR快速检测方法。实验证明该方法具有良好的特异性与敏感性,对维氏气单胞菌基因组DNA的最低检测浓度为1.58×10-4ng;在人工模拟试验的30份样品中:使用PCR检测方法的样本检出率达到了86.7%(26/30),高于细菌分离培养的检出率73.3%(22/30)。该方法的建立为维氏气单胞菌的快速检测提供了新的方法。
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| 关 键 词： | 维氏气单胞菌 聚合酶链式反应 核酸酶 |
| 收稿时间： | 8/1/2012 5:13:03 PM |
| 修稿时间： | 2012/10/30 0:00:00 |
Establishment and Preliminary Application of Rapid Detection Method for Aeromonas Veronii by PCR |
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| BIAN Yu,QIAN Hong-wei,MENG Qing-feng,KANG Yuan-huan,HE De-cong,SHAN Xiao-feng,WANG Wei-li,QIAN Ai-dong.Establishment and Preliminary Application of Rapid Detection Method for Aeromonas Veronii by PCR[J].Chinese Journal of Veterinary Drug,2013,47(1):1-3. |
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| Authors: | BIAN Yu QIAN Hong-wei MENG Qing-feng KANG Yuan-huan HE De-cong SHAN Xiao-feng WANG Wei-li QIAN Ai-dong |
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| Institution: | College of Animal Science and Technology of Jilin Agricultural University, |
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| Abstract: | Rapid Detection Method by PCR for Aeromonas Veronii was established by nuclease gene regarded as the target gene in this study. The results showed that the method had good specificity and sensitivity and the minimum detectable concentration to A.veronii was 1.58 ? 10-4 ng. Trials of artificial simulated pollution showed that the detectable rate of this method reached 86.7% and it was higher than the rate of 73.3% by bacteria cultivation. The establishment of the method provides a new way for rapidly detecting A.veronii.. |
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| Keywords: | Aeromonas Veronii PCR Nuclease |
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