| 黄芪皂苷三种提取方法对黄芪甲苷含量的影响 |
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| 引用本文： | 徐先祥.黄芪皂苷三种提取方法对黄芪甲苷含量的影响[J].中国兽药杂志,2008,42(4):12-14. |
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| 作者姓名： | 徐先祥 |
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| 作者单位： | 安徽中医学院第一附属医院药学部,安徽合肥230031 |
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| 基金项目： | 安徽省自然科学基金
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国家自然科学基金 |
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| 摘 要： | 用薄层扫描法测定并比较三种不同皂苷提取方法对黄芪甲苷含量的影响。分别采用大孔树脂吸附法、正丁醇萃取法和KOH回流提取法制备供试品。以氯仿-甲醇-水为展开系统，薄层扫描法测定黄芪甲苷含量。结果显示，大孔树脂吸附法、正丁醇萃取法和KOH回流提取法三种方法提取物中黄芪甲苷平均含量分别为0．07768％，0．05578％，0．06953％。结果显示大孔树脂法提取黄芪所得黄芪甲苷含量较高。
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| 关 键 词： | 黄芪皂苷 提取 黄芪甲苷 含量测定 薄层扫描法 黄芪皂苷 提取物 方法 黄芪甲苷含量 影响 Astragaloside Content Methods Saponin Astragalus 树脂法 大孔树脂吸附法 平均含量 显示 结果 系统 展开 甲醇 氯仿 法制 |
| 文章编号： | 1002-1280(2008)04-0012-03 |
| 收稿时间： | 2008/1/11 0:00:00 |
| 修稿时间： | 2008年1月11日 |
Effects of Three Saponin Extracting Methods on Content of Astragaloside IV |
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| XUXIANXIANG.Effects of Three Saponin Extracting Methods on Content of Astragaloside IV[J].Chinese Journal of Veterinary Drug,2008,42(4):12-14. |
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| Authors: | XUXIANXIANG |
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| Institution: | China Pharmceutical University |
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| Abstract: | Objective To determine content of astragaloside IV by TLC-scanning in three extraction with different methods. Methods Three methods including D101 macroreticular resin method, extraction refine by n-butyl alcohol and KOH-regurgitate pheresis were applied to Astragalus saponins extracting, contents of astragaloside IV were determined by TLC-scanning. The mobile phase was chloroform-methanol-water. Results The average contents of Astragaloside IV was 0.07768% by D101 macroreticular resin method, 0.05578% by extraction refine by n-butyl alcohol and 0.06953% by KOH-regurgitate pheresis. Conclusions The method of macroreticular resin is better than two others. |
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| Keywords: | Astragalus saponins extracting Astragaloside IV content determination TLC-scanning |
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