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| 间接ELISA法检测鸡新城疫抗体 | |
| 引用本文： | 黄金海,刘业兵,丁伯良,张健,王东.间接ELISA法检测鸡新城疫抗体[J].中国兽药杂志,2005,39(6):22-26. |
| 作者姓名： | 黄金海 刘业兵 丁伯良 张健 王东 |
| 作者单位： | 1. 天津大学农业与生物工程学院,天津,300072;天津市畜牧兽医研究所,天津,300112 2. 中国兽医药品监察所,北京,100081 3. 天津市畜牧兽医研究所,天津,300112 |
| 基金项目： | 天津市自然科学基金，天津大学校科研和教改项目 |
| 摘 要： | 应用醛化的鸡红细胞经吸附释放方法获得纯化的新城疫病毒(NDV),经triton X-100处理并反复冻融制备新城疫 ELISA抗原.应用抗鸡Ig轻链单抗1B7辣根过氧化物酶结合物建立了定量检测NDV抗体水平的ELISA方法.确立了将鸡血清100倍稀释检测ELISA效价(ET)的回归方程y=0.941 28 x 0.206 77,r=0.981 63,可用于定量测定.血凝抑制(HI)方法与ELISA方法的比较试验表明,ELISA法的敏感性是HI方法的3倍以上.应用建立的ELISA方法比较了卵黄、气管及胆汁中局部抗体与血清抗体的差异. |
| 关 键 词： | 鸡新城疫 抗体 |
| 文章编号： | 1002-1280(2005)06-0022-05 |
| 修稿时间： | 2005年1月24日 |
Indirect ELISA method for detecting the antibody to newcastle disease virus of chickens |
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| HUANG Jin-hai,LIU Ye-Bing,DING Bo-liang,ZHANG Jian,WANG Dong.Indirect ELISA method for detecting the antibody to newcastle disease virus of chickens[J].Chinese Journal of Veterinary Drug,2005,39(6):22-26. | |
| Authors: | HUANG Jin-hai LIU Ye-Bing DING Bo-liang ZHANG Jian WANG Dong |
| Abstract: | The indirect ELISA method for detecting antibody level to newcastle disease virus was developed by preparing coating antigen by absorbing and releasing virus from chicken blood cells, and enzyme conjugate of mouse against light chain of chicken Ig. A linear regression equation for detecting titer was developed under 100 fold dilution sera sample that was y=0.941 28 x+0.206 77,r=0.981 63. The ELISA titer was 3 times higher than that of hemagglutination inhibitor test (HI).Using ELISA method, the NDV antibody titre in sera was compared with that in yolk, bile, and tracheal, respectively. |
| Keywords: | ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |