茶皂素对PRRSV感染Marc-145细胞受体表达及Caspase-9活化的影响

以茶皂素(Teasaponin,TS)作为候选药物,研究茶皂素对Marc-145细胞受体CD163和波形蛋白(Vimentin)基因合成和蛋白表达的影响,以及茶皂素是否能通过细胞凋亡内源性通路影响PRRSV感染细胞,探究茶皂素抗PRRSV的作用机制。通过qRT-PCR和Western blot检测TS对细胞受体CD163和Vimentin的基因合成和蛋白表达的影响。运用Western blot技术检测TS对细胞内源性凋亡通路启动子caspase-9活化的影响,初探TS的抗PRRSV机制。qRT-PCR结果表明TS能显著抑制感染PRRSV的Marc-145细胞受体CD163和Vimentin基因的合成。Western blot结果表明TS能显著抑制细胞受体CD163和Vimentin的蛋白表达。TS能够引起细胞内源性凋亡通路启动子caspase-9的活化。研究表明,TS能抑制PRRSV在Marc-145细胞上的穿入过程,从而达到抗PRRSV的作用;亦可通过激活细胞凋亡内源性通路以早期促进细胞凋亡的方式产生抗PRRSV的作用。

Effects of tea saponin on PRRSV related cell receptors and caspase-9 activation in Marc 145 cells

(Objective)Taking tea saponin (TS) as the candidate drug of anti-PRRSV, the antiviral mechanism of TS was explored through the determination of the effect on the Marc-145 receptor CD163 and Vimentin gene synthesis and protein expression, as well as whether could affect PRRSV infection by cell endogenous apoptotic pathway. (Methods)qRT-PCR and Western blot technologies were used to detect the influence of TS on cell receptor CD163 and Vimentin gene synthesis and protein expression. In addition, the effect of TS on the caspase-9 activation which is cell endogenous apoptotic pathway promoter was determined by Western blot. (Results)qRT-PCR results showed that TS could significantly inhibit the gene synthesis of CD163 and Vimentin in the PRRSV infected Marc-145 cells. Moreover, Western blot results showed that TS could significantly inhibit the expression of CD163 and Vimentin and induce caspase-9 activation.(Conclusion) TS could significantly inhibit PRRSV enter into Marc-145 cells and activate the cell endogenous apoptotic pathway to induce the early apoptosis to anti-PRRSV replication.