| ELISA检测兔巴氏杆菌病抗体的研究 |
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| 引用本文： | 郭明璋,王启明.ELISA检测兔巴氏杆菌病抗体的研究[J].中国兽药杂志,1997,31(1):1-5. |
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| 作者姓名： | 郭明璋 王启明 |
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| 作者单位： | 江苏省农业科学院畜牧兽医研究所 |
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| 摘 要： | 应用ＥＤＴＡ处理和超声波裂解法提取多杀性巴氏杆菌的荚膜多糖和外膜蛋白抗原，包被酶标板，用ＨＲＰ－ＳＰＡ代替酶标抗体，建立了检测兔多杀性巴氏杆菌病抗体的间接法ＥＬＩＳＡ。试验确定抗原的包被浓度为６．０５μｇ／ｍｌ，ＨＲＰ－ＳＰＡ的适宜稀释度为１１００００，血清ＥＬＩＳＡ滴度在１４００以上判为阳性。测定了多杀性巴氏杆菌灭活菌苗免疫兔血清１０份，均呈阳性，抗体滴度在１１２８０～１４０９６０范围内呈正态分布；血清几何平均效价为１５１２０。重复４次测定１０份阳性血清，其ＯＤ值之标准差（ＳＤ）＜０．１０，变异系数（ＣＶ）＜１０．０％。本法与琼脂扩散沉淀试验相比，敏感度提高约１５７５倍。ＥＬＩＳＡ不仅敏感、特异、快速、简便，而且经济、易于重复，适于推广应用。
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| 关 键 词： | 多杀性巴氏杆菌荚 膜多糖抗原 外膜蛋白抗原 抗体 ELISA 检测 |
An Indirect Enzyme linked Immunosorbent Assay for Detection of Antibody against Pasteurellosis |
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| Guo Mingzhang,Wang Qiming,Dong Yafang,Xue Jiabin,Xu Weizhong Institute of Animal Husbandry and Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing.An Indirect Enzyme linked Immunosorbent Assay for Detection of Antibody against Pasteurellosis[J].Chinese Journal of Veterinary Drug,1997,31(1):1-5. |
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| Authors: | Guo Mingzhang Wang Qiming Dong Yafang Xue Jiabin Xu Weizhong Institute of Animal Husbandry and Veterinary Medicine Jiangsu Academy of Agricultural Sciences Nanjing |
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| Institution: | Guo Mingzhang,Wang Qiming,Dong Yafang,Xue Jiabin,Xu Weizhong Institute of Animal Husbandry and Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014 |
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| Abstract: | |
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| Keywords: | Pasteurella multocida capsule polysaccharide and outer membrane protein of pasteurella multocida antibody ELSA detection |
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