| 鸡传染性贫血病毒VP1、VP2基因的表达及免疫原性研究 |
| |
| 引用本文： | 徐微,杨承槐.鸡传染性贫血病毒VP1、VP2基因的表达及免疫原性研究[J].中国兽药杂志,2015,49(4):7-11. |
| |
| 作者姓名： | 徐微 杨承槐 |
| |
| 作者单位： | 中国兽医药品监察所,中国兽医药品监察所 |
| |
| 基金项目： | 科技部科技基础性工作专项“重大动物疫病病原及相关制品标准物质研究（2008FY130100）” |
| |
| 摘 要： | 为探索鸡传染性贫血重组蛋白亚单位疫苗的可行性,将鸡传染性贫血病毒基因分别克隆到转移载体p Fast Bac HTA中,再将其分别转化DH10Bac感受态细胞得到相应的重组表达载体,转染昆虫细胞Sf21获得含有VP1、VP2基因的重组杆状病毒v Bac-VP1、v Bac-VP2,应用悬浮培养的Sf21细胞表达重组蛋白。SDS-PAGE及Western blotting结果证明,VP1、VP2基因在昆虫细胞中得到了表达,动物试验进一步证实,重组蛋白免疫SPF鸡可产生ELISA抗体,提示杆状病毒表达的VP1、VP2具有良好的免疫原性。
|
| 关 键 词： | 鸡传染性贫血病毒 杆状病毒表达 VP1 VP2 免疫原性 |
| 收稿时间： | 2014/10/10 0:00:00 |
| 修稿时间： | 2015/1/16 0:00:00 |
Expression of VP1 and VP2 genes of chicken anemia virus and Immunogenicity Research |
| |
| Institution: | china institute of veterinary drug control, |
| |
| Abstract: | The VP1 and VP2 genes of chicken anemia virus were cloned into pFastBacHTA vectors separately, then transform into competent cells of E. coli DH10Bac. The recombinant baculoviruses vBac-VP1 and vBac-VP2 were generated by transfection of recombinant bacmid into Sf21 insect cells. Finally, recombinant proteins were expressed in suspension culture Sf21 cells. SDS-PAGE and Western blotting showed that VP1 and VP2 have been expressed in Sf21 cells. Immunized the SPF chicken with recombinant protein, their serum were inspected by ELISA and showed good immunogenicity. |
| |
| Keywords: | chicken anemia virus baculovirus expression VP1 VP2 immunogenicity |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国兽药杂志》浏览原始摘要信息 |
| 点击此处可从《中国兽药杂志》下载免费的PDF全文 |
