微生物浊度法测定3种兽用抗生素效价的研究

分别以金黄色葡萄球菌和大肠杆菌为试验菌，抗生素检定培养基Ⅲ号为试验用培养基，制成菌悬液。将硫酸安普霉素、泰乐菌素、硫酸黏菌素稀释成不同的浓度加入菌悬液中，37℃培养3～4h，确立浓度的对数值与相对应的吸光度呈直线相关的线性范围，并对所建立的浊度法进行准确度、精密度、浊度法与管碟法比较及专属性等验证。结果表明硫酸安普霉素、泰乐菌素、硫酸黏菌素的线性浓度范围分别为2．9—7．2、0．6～2．0、44．9～93．2u／mL，相关系数r分别为0．9974、0．9984、0。9978。硫酸安普霉素、泰乐菌素、硫酸黏菌素所建立的浊度法平均回收率及平均可信限率分别为99．82％，2．48％、99．66％，2．80％、99．61％，7．62％。管碟法与浊度法所测结果无显著差异。硫酸安普霉素和泰乐菌素浊度法具有可信限率低、灵敏度高、精密度高、快速等优点；硫酸黏菌素建立的浊度法可信限率与管碟法接近。

Determination of the Potency of antibiotics by Turbidimetric Method

Turbidimetry assay for fast determination of apramycin sulfate, tylosin, colistin sulfate were developed. Staphylococcus aureus and E. coli as test organisms and antibiotics standardization medium Ⅲ as test medium were used. At the range of 2.9 - 7.2 u/mL for apramyein sulfate,0.6 - 2.0 u/mL for tylosin, and 44.9 - 93.2 u/mL for colistin sulfate, the standard curve was linear with r of 0. 997 4, 0. 998 4 and 0. 9978 respectively. The turbidimetry assay of apramycin sulfate and tylosin were accurate and fast, while the cofidence limit of turhidimetry assay of colistin sulfate was relative higher.