固相萃取-UPLC-MS/MS快速测定猪尿中的苯乙醇胺A

建立了-种固相萃取-超高效液相色谱-串联质谱法（SPE—UPLC—MS／MS）快速测定猪尿中苯乙醇胺A的方法。猪尿样品以莱克多巴胺-D，为内标，尿液经酸化后离心，上清液经OasisMCX固相萃取柱净化，超高效液相色谱-串联质谱法测定。苯乙醇胺A监测离子为m／z345〉150和m／z 345〉327。结果表明在0．2～5μg／L范围内苯乙醇胺A呈良好的线性关系，高中低浓度绝对回收率在80—110％，精密度小于15％，最低定量限0．2μg／L。本方法快速、简便，适于猪尿中苯乙醇胺A的残留测定。

Rapid determination of phenylethanolamine A in pig urine by SPE-UPLC-MS/MS

A SPE-UPLC-MS/MS method was developed and established to rapidly determine phenylethanolamine A in pig urine. The urine sample was acidified and centrifuged, and the supernatant was cleaned up by Oasis MCX. UPLC-MS/MS was implemented to detect phenylethanolamine A by m/z 345>150 and m/z 345>327. The results illustrated that good linearity in the ranges of 0.2~5 mg/L for phenylethanolamine A. The absolute recoveries were between 80 % and 110 %, and precision was lower than 15 %. The limit of quantitative detection was 0.2 mg/L. The method was simple and rapid, and it was suitable to detect phenylethanolamine A residual in pig urine samples.