HPLC法测定徐长卿中绿原酸的含量

建立HPLC法测定徐长卿中绿原酸的含量。采用索氏提取法以70％的甲醇水溶液为提取剂对徐长卿中的绿原酸进行提取。在SHIMADZU C18色谱柱（250mm×4．6mm，5μm），流动相乙腈-0．5％磷酸水溶液（1：9，V/V）；流速1．0mL/min；柱温25℃；检测波长327nm的条件下进行检测。结果表明，绿原酸在0．232—4．640μg范围内与峰面积呈良好线性关系，r=0．9997。平均回收率为99．32％，RSD为0．216％（n=5）。该方法简便、可靠、重现性好，可作为徐长卿中绿原酸含量测定的方法。

Determination the Contents of Chlorogenic acid in Cynanchum paniculatum (Bge.) Kitag. with HPLC

An HPLC method for determination the content of chlorogenic acid in Cynanchum paniculatum （ Bge. ） Kitag was established, Using 70% methyl alcohol to extract chlorogenic acid from Cynanchum paniculatum （Bge.） Kitag by Soxhlet extraction method. The determination was measured by HPLC with SHIMADZU - C18 （250 mm x 4.6 mm,5μm）column, acetonitrile- 0.5% H3PO4 （1：9, V/V） as mobile phase. The flow rate was 1.0 mL/min,detection wavelength was 327 nm and column temperature was 25℃. The results showed that the linear range of chlorogenic acid was 0. 232 - 4.640 μg, r = 0. 999 7. The average recovery was 99.32% with RSD O. 216% （n = 5）. This method was simple and reliable with good reproducibility which could be used to determine chlorogenic acid in Cynanchum paniculatum （Bge.） Kitag.