超高效液相色谱-串联质谱法测定猪肉中巴喹普林残留

建立了猪肉中巴喹普林药物残留的超高效液相色谱一串联质谱测定方法，样品用乙酸乙酯提取，吹干后用60％乙腈水（0．1％甲酸）溶解，供液相色谱一串联质谱法分析，内标法定量。结果表明：巴喹普林标准溶液在100～800μg／L范围内呈现良好线性，r2均大于0．998；方法检出限为5μg／kg，定量限为10μg／kg；巴喹普林在10～80μg／kg添加范围内的平均回收率为90．8％-108％，批内、批间RSD均小于6．9％。该方法简便快捷、灵敏度高、定性准确，能满足食品安全检测右吴浩妾田．的霉求．

determination of Baquiloprim residues in Pig muscle by UPLC-MS/MS

An uhral performance liquid chromatography - tandem mass spectrometry （ UPLC - MS/MS） method was established for the determination of baquiloprim residues in pig muscle. The samples were extracted by Ethyl acetate, dissolved by 60% acetonitril （0. 1% Formic acid ） and determinatied on UPLC - MS/MS After evaporation. The method was quantified by Internalstandard method. The calibration curves were linear between the peak areas and the concentrations of 20 - 800 μg/L with correlation coefficients （r2） more than 0.998. The limit of detection of baquiloprim was 5 μg/kg, and the limit of quantitation was 10 μg/kg. The average recoveries from spiked muscle at concentrations of 10 - 80 μg/kg ranged 90. 8% - 108% and the relative standard deviations of intra - and inter - batch were both less than 6.9%.