双葛止泻口服液抑菌剂用量筛选与抑菌效力研究

通过抑菌效力研究确定了双葛止泻口服液中抑菌剂的合理添加量。根据《中国兽药典》2015年版一部附录“1121抑菌效力检查法”要求,以金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、白色念珠菌和黑曲霉为试验菌株,建立双葛止泻口服液抑菌效力检查方法,对不同苯甲酸钠含量的双葛止泻口服液进行抑菌效力测定,筛选出供试品中抑菌剂合理添加量。结果表明,0.3%的苯甲酸钠对微生物生长繁殖具有良好的抑制作用,且为最低有效剂量。因此,0.3%的苯甲酸钠可作为双葛止泻口服液的抑菌剂用量。

Study on dosage screening of antimicrobial preservatives and antimicrobial effectiveness of Shuangge Zhixie Oral Liquid

The reasonable addition amount of the bacteriostatic agent in Shuangge Zhixie Oral Liquid was determined by studying the antimicrobial effectiveness.According to the appendix 1121(method for antibacterial efficacy test)of Chinese Veterinary Pharmacopoeia(2015 edition,volumeⅠ),and Staphylococcus aureus,Pseudomonas aeruginosa,Escherichia coli,Candida albicans and Aspergillus niger as test strains,method for antibacterial efficacy test of Shuangge Zhixie Oral Liquid was established.The antibacterial efficacy of Shuangge Zhixie Oral Liquid with different sodium benzoate content was tested,and the reasonable addition amount of the antimicrobial preservative added to the test product was screened out.Results showed that the growth of test microorganisms were inhibited effectively by 0.3%sodium benzoate added to Shuangge Zhixie Oral Liquid which was also the minimum effective dosage.0.3%sodium benzoate could be used as antimicrobial preservative for Shuangge Zhixie Oral Liquid.