猪链球菌Ⅱ型四川分离株对抗菌药的敏感性分析

对四川省不同地区链球菌病病死猪体内分离的7株链球菌Ⅱ型进行抗菌药敏感性试验,用链球菌兰氏D群C55914作为对照,比对菌为肺炎链球菌质控菌株ATCC49619.在32种抗菌药中,选择29种药物进行纸片法药敏试验.试验结果表明:7株链球菌耐药谱非常相似,对青霉素、氨苄西林、阿莫西林、奥格门丁、卡那霉素、红霉素、壮观霉素、氯霉素、氟苯尼考、头孢氨苄、头孢拉定、头孢他定、头孢呋辛、头孢曲松、环丙沙星、氧氟沙星、二氟沙星、沙拉沙星、达氟沙星、恩诺沙星、复方新诺明、万古霉素、亚安培南均敏感;对阿米卡星、新霉素、庆大霉素3株敏感,4株中敏;对四环素和多西环素2株中敏,5株耐药;对链霉素1株中敏,6株耐药.用21种药物进行最小抑菌浓度(MIC)测定,结果7株均对青霉素、氨苄西林、卡那霉素、红霉素、头孢他定、头孢呋辛、头孢曲松、头孢噻呋、环丙沙星、达氟沙星、氧氟沙星、二氟沙星、沙拉沙星、恩诺沙星、磺胺六甲氧嘧啶敏感,1株对新霉素和阿米卡星中敏,2株耐药;所有菌株对四环素、土霉素、多西环素、链霉素均耐药.两种方法总体结果一致.

Analysis for antimicrobial susceptibility of Streptococcus suis isolated from Sichuan Province

Antimicrobial susceptibility test for 7 stains of Streptococcus suis isolated from sick and dead swine of different areas in Sichuan province have been done with Streptococcus C55914(lance-field D sero-group) as(control) and Streptococcus pneumoniae as quality control.29 antimicrobials were used for disk diffusion test and 21(antimicrobials) were used for minimal inhibitory concentration(MIC) tests.The results of disk diffusion test(indicated) the drug-resistance profile of 7 stains of Streptococcus suis were conform.All of isolates were susceptive to penicillin,ampicillin,amoxicillin,amoxicillin-clavulanic acid,kanamycin,erythromycin,spectinomycin,chloramphenicol,florfenicol,cefalexin,cefradine,ceftazidime,cefuroxime,ceftriaxone,ciprofloxacin,ofloxacin,difloxacin,saraftoxacin,danofloxacin,enrofloxacin,trimethoprim-sulfamethoxazole,vancomycin,imipenen,4(isolates) were susceptive and 3 isolates were intermediate to amikacin,neomycin,gentamicin.2 isolates were(intermediate) and 5 isolates were resistant to tetracycline and doxycycline.A isolate was intermediate and 6 isolates were resistant to streptomycin.The results of MIC test indicated that all isolates were susceptive to penicillin,(ampicillin),kanamycin,erythromycin,ceftazidime,cefuroxime,ceftriaxone,ceftiofur,ciprofloxacin,ofloxacin,difloxacin,sarafloxacin,danofloxacin,enrofloxacin,sulfamethoxydiazine.A isolate was intermediate and 2(isolates) were resistant to neomycin and amikacin.All of isolates were resistant to tetracycline,oxytetracycline,doxycycline and streptomycin.It shows the similar results with two methods.