| 不同细胞培养基细胞培养动力学比较试验 |
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| 引用本文： | 吴长新,焦新安.不同细胞培养基细胞培养动力学比较试验[J].中国兽药杂志,1997,31(3):12-15. |
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| 作者姓名： | 吴长新 焦新安 |
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| 作者单位： | 扬州大学畜禽传染病农业部部级重点实验室，江苏省生物工程重点实验室 |
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| 摘 要： | 应用宜兴赛尔生物化工厂产１９９、Ｆ－１０、１６４０和ＤＭＥＭ细胞培养基与美国Ｓｉｇｍａ公司产１９９、Ｆ－１０、１６４０和ＤＭＥＭ细胞培养基分别配制细胞培养液，培养ＳＰ２／０和Ｖｅｒｏ细胞系及原代鸡胚成纤维细胞（ＣＥＦ），做细胞培养动力学比较试验。比较这两种来源的细胞培养基对细胞生长的形态、分裂速度及鸡马立克氏病毒（Ｍａｒｅｋ＇ｓｄｉｓｅａｓｅｖｉｒｕｓ．ＭＤＶ）疫苗毒株ＨＶＴ－ＦＣ１２６和ＲｉｓｐｅｎｓＣＶＩ９８８在ＣＥＦ单层上增殖量的差异。研究结果表明，四种国产细胞培养基与对应的四种进口培养基对细胞生长及病毒增殖的影响无显著差异
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| 关 键 词： | 细胞培养基 细胞生长动力学 比较试验 |
Comparative Trails of Dynamics of Cell Culture in Different Cell Media |
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| Wu Changxin,Gan Junji,Jiao Xinan ,Liu Jinbiao Key Lab. of Animal Infectious Disease of Agriculture Ministry,Yangzhou University,Yangzhou, Key Lab. of Bioengineering of Jiangsu Province,Yangzhou University,Yangzhou.Comparative Trails of Dynamics of Cell Culture in Different Cell Media[J].Chinese Journal of Veterinary Drug,1997,31(3):12-15. |
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| Authors: | Wu Changxin Gan Junji Jiao Xinan Liu Jinbiao Key Lab of Animal Infectious Disease of Agriculture Ministry Yangzhou University Yangzhou Key Lab of Bioengineering of Jiangsu Province Yangzhou University Yangzhou |
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| Institution: | Wu Changxin,Gan Junji,Jiao Xinan 1,Liu Jinbiao 1 Key Lab. of Animal Infectious Disease of Agriculture Ministry,Yangzhou University,Yangzhou,225009 1Key Lab. of Bioengineering of Jiangsu Province,Yangzhou University,Yangzhou,2250 |
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| Abstract: | Comparative trails were made with native manufactured and imported cell media to evaluate difference of dynamics of cell culture.CEF,SP2/0 line and Vero line were cultured in different cell media named 199,F 10,1640 and DMEM manufactured by Yixin Saier Biochemistry Company in China and Sigma Biochemistry Company in America respectively. Mareks disease virus (MDV) HVT Fc126 vaccine strain was inoculated in CEF monolayer cultured in different celll media. The results of the trails showed that four kinds cell media manufactured by the two company could supply sufficient nutrient for cell growth and the two orignated cell media did not affect cell line morphology and ratio of cell line multiplying and mulriplying quantity of the virus inoculated in CEF monolayer significantly. |
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| Keywords: | cell medium dynamics of cell growth comparative trail |
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