2017-2021年上海市动物源粪肠球菌、屎肠球菌耐药性情况变迁分析

为了解上海地区动物源粪肠球菌、屎肠球菌对常见抗菌药物的耐药及其最小抑菌浓度(minimal inhibitory concentration,MIC)变迁情况，采用微量肉汤稀释法对近五年采集的粪肠球菌、屎肠球菌进行10种常见抗菌药物敏感性测试。结果表明，458株粪肠球菌及283株屎肠球菌对头孢西丁、头孢噻呋及氧氟沙星耐药率较高(均高于60%)，对青霉素、阿莫西林/克拉维酸及万古霉素的耐药率较低(均低于11%)，粪肠球菌耐药率整体高于屎肠球菌，两者对氧氟沙星、头孢噻呋、氟苯尼考、庆大霉素、利奈唑胺、恩诺沙星的耐药率存在较大差异（耐药率差异为15%~44%）。五年间，粪肠球菌对阿莫西林/克拉维酸、氧氟沙星的MIC50及MIC90均呈下降趋势；屎肠球菌对阿莫西林/克拉维酸、头孢西丁及氟苯尼考的MIC50及MIC90均呈下降趋势。研究表明，近年来上海地区动物源粪肠球菌、屎肠球菌的耐药情况有好转趋势，仍需继续加强肠球菌耐药性监测。

Analysis on the Change of Drug Resistance of Enterococcus faecalis and Enterococcus faecium from Animals in 2017-2021 in Shanghai

To understand the resistance of Enterococcus faecalis and Enterococcus faecium from animals to common antibiotics and the changes of minimum inhibitory concentration (MIC) in Shanghai, the micro broth dilution method was used to test the susceptibility of Enterococcus faecalis and Enterococcus faecium which collected in the past five years for 10 common antibacterial drugs.The results showed that 458 strains of Enterococcus faecalis and 283 strains of Enterococcus faecium were highly resistant to cefoxitin, ceftiofur and ofloxacin(all above 60%), low resistance to penicillin, amoxicillin/clavulanate, and vancomycin (all less than 11%). The drug resistance rate of Enterococcus faecalis was generally higher than that of Enterococcus faecalis. The resistance rates of them to ofloxacin, ceftiofur, florfenicol, gentamicin, linezolid, and enrofloxacin were significantly different (the difference in the resistance rates was 15%~44%). The MIC50 and MIC90 of Enterococcus faecium to amoxicillin/clavulanate, cefoxitin and florfenicol also showed a decreasing trend.Studies have shown that the drug resistance of Enterococcus faecalis and Enterococcus faecium from animals in Shanghai has improved in recent years, and it is still necessary to continue to strengthen the monitoring of drug resistance of Enterococcus.