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高效液相色谱柱前衍生化法测定饲料中的盐霉素
引用本文:马立农,陈杖榴. 高效液相色谱柱前衍生化法测定饲料中的盐霉素[J]. 中国兽药杂志, 2002, 36(2): 33-35
作者姓名:马立农  陈杖榴
作者单位:1. 深圳职业技术学院生物应用工程系,广东广州,510642
2. 华南农业大学兽医药理学研究室,广东广州,510642
摘    要:以甲醇∶1 5 %醋酸水溶液 (90∶10 ,v/v)为流动相 ,2 ,4 二硝基苯肼 (DNP)为衍生剂 ,应用高效液相色谱柱前衍生化法 ,采用紫外检测方法对饲料中的盐霉素进行了测定。盐霉素标准品的保留时间为 8 99min ,饲料中盐霉素的保留时间为 8 91min。经测定盐霉素标准液的标准曲线回归方程与相关系数 ,其线性关系理想 ,重现性好。采用此方法测得的总回收率为 89 0 6% ,总变异系数为 1 73%。此方法的检测限为 1mg/kg。

关 键 词:高效液相色谱法  柱前衍生化  盐霉素  饲料
文章编号:1002-1280(2002)02-0033-03
修稿时间:2001-08-31

Liquid Chromatography Detection of Monensin in Poultry Feeds Using Pre-column Derivatization
MA Li nong ,CHEN Zhang liu. Liquid Chromatography Detection of Monensin in Poultry Feeds Using Pre-column Derivatization[J]. Chinese Journal of Veterinary Drug, 2002, 36(2): 33-35
Authors:MA Li nong   CHEN Zhang liu
Affiliation:MA Li nong 1,CHEN Zhang liu 2
Abstract:An effective analytical procedure of liquid chromatography with ultraviolet detection of monensin in poultry feeds using pre column derivatization was tested. The derivatization mixture, which was derivatized with 2,4 dinitrophenylhydrazine (DNP), was analyzed with methanol-1.5% aqueous acetic acid (90:10, v/v ) as eluent. The RT of standard monensin was 8.99 minutes, and that of monensin in poultry feeds was 8.91 minutes. The data reported showed that the method had good linearity and repeatability results. The total recovery for monensin was 89.06%, and relative standard deviation was 1.73%. The detection limit of the method was 1 mg/kg.
Keywords:HPLC  pre column derivatization  monensin  poultry feeds  
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