| 仔猪小肠上皮细胞刷状缘膜囊制备方法的研究 |
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| 引用本文： | 代建国,李德发,朴香淑,龚利敏,丁玉华.仔猪小肠上皮细胞刷状缘膜囊制备方法的研究[J].中国畜牧杂志,2004,40(7):6-8. |
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| 作者姓名： | 代建国 李德发 朴香淑 龚利敏 丁玉华 |
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| 作者单位： | 中国农业大学国家饲料工程技术研究中心,北京,100094 |
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| 摘 要： | 采用CaCl2 和MgCl2 两种沉淀法制备了断奶仔猪小肠上皮细胞刷状缘膜囊 (BBMV) ,并通过测定BBMV制备过程中的细胞膜或细胞器标志酶酶活、核糖核酸和脱氧核糖核酸含量等指标对所制备的BBMV的纯度进行了评价。结果表明采用MgCl2 沉淀方法制备的断奶仔猪小肠BBMV纯度优于CaCl2 沉淀法。
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| 关 键 词： | 畜牧学 小肠 刷状缘膜囊 制备 仔猪 |
| 文章编号： | 0258-7033(2004)07-0006-03 |
| 修稿时间： | 2004年4月8日 |
Study on Preparation of Small Intestinal Brush Border Membrane Vesicles of Piglets |
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| DAI Jian-guo,LI De-fa,PIAO Xiang-shu,GONG Li-min,DING Yu-hua.Study on Preparation of Small Intestinal Brush Border Membrane Vesicles of Piglets[J].Chinese Journal of Animal Science,2004,40(7):6-8. |
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| Authors: | DAI Jian-guo LI De-fa PIAO Xiang-shu GONG Li-min DING Yu-hua |
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| Abstract: | Effect of different precipitation methods (CaCl_2 and MgCl_2) on the purity of small intestinal BBMV were investigated in this study. Purification of the BBMV was routinely checked by determination of the cell membrane and organelle marker enzyme activities, Deoxygenrobose Nucleic Acid (DNA) content and Robose Nucleic Acid (RNA) content. The result indicated that magnesium chloride aggregation method was better to prepare the weaned piglet small intestine BBMV than calcium chloride aggregation method. |
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| Keywords: | Animal science Small intestine Brush border membrane vesicles Preparation Piglets |
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