撒坝猪血清淀粉酶多态性与生长速度关系的研究

本研究采用垂直板聚丙烯酰胺凝胶电泳法对１１５头撒坝猪血清淀粉酶（Ａｍ）多态性进行了检测，并用二因素有互作的最小二乘模型对不同淀粉酶基因型与生长速度的关系进行了分析。结果表明，所检测到的血清淀粉酶ＡＡ、ＡＢ和ＢＢ３种基因型的频率分别为０．１７３９、０．４２６１和０．４０００，两个等位基因Ａ和Ｂ的频率分别为０．３８７０和０．６１３０，未发现Ｃ基因。该位点的遗传多样性指数为０．４７４４。在３种基因型中，ＢＢ型个体的６月龄体重、２～６月龄日增重和４～６月龄日增重最高，且淀粉酶基因型与性别之间在２０日龄体重、４月龄体重、６月龄体重、０～２０日龄日增重、２～４月龄日增重、２～６月龄日增重和４～６月龄日增重等性状上存在着明显的互作效应，显示出血清淀粉酶多态性作为增重速度的遗传标记以进行标记辅助选择的可能性

Study on the Relationship of Serum Amylase Polymorphism and Growth Traits of Saba Pig

The serum amylase polymorphism of 115 Saba pigs was investigated by using the method of vertical polyacrylamide gel electrophoresis and the relationship of amylase genotypes and growth traits was analyzed by the least square model of two factors(amylase genotype and sex)with interaction. The results showed that the frequencies of genotype AA,AB and BB were 0.1739,0.4261 and 0.4000 respectively,and the frequencies of allele A and B were 0.3870 and 0.6130 respectively. The allele C was not found in this study. The index of genetic diversity of this locus was 0.4744. Among the three genotypes,the 6 months body weight,the daily gains of 2-6 months and 4-6 months of genotype BB were significantly better than those of the other two genotypes. The interaction effects of amylase genotype and sex on the body weights of 20 days,4 months and 6 months and the daily gains of 0-20 days,2-4 months,2-6 months and 4-6 months were significant. It indicated the possibility of using serum amylase polymorphism as the genetic marker of pig growth traits in marker assisted selection.