复合酸化剂对断奶仔猪肠黏膜形态和肠道微生物区系及免疫功能的影响

试验选取200头仔猪分为5组,每组4个重复。分别为饲喂常规日粮(对照组)、添加0.1%磷酸型酸化剂A、0.3%磷酸型酸化剂A、0.1%乳酸型酸化剂B和0.3%乳酸型酸化剂B日粮。于试验第28天早上每个处理选择3头健康仔猪进行屠宰,测定仔猪小肠黏膜形态、大肠微生物菌群及免疫指标。结果表明:添加所研制的磷酸型和乳酸型酸化剂可以显著增加小肠绒毛高度(P<0.05);添加磷酸型酸化剂A和乳酸型酸化剂B都可以显著降低仔猪盲肠大肠杆菌和沙门氏菌的数量(P<0.05),增加乳酸菌和双歧杆菌的数量(P<0.05),其中添加乳酸型酸化剂B的效果显著优于磷酸型酸化剂A(P<0.05);添加0.3%乳酸型酸化剂B可以显著降低仔猪血清中尿素氮的含量(P<0.05);添加2种酸化剂都可以显著提高仔猪血清中IgA、IgG的浓度(P<0.05)。

Effects of Acidifiers on Small Intestine Morphology, Intestinal Microbial Flora and Immune Response of Weaned Pigs

Two-hundred weaned pigs were divided into five groups randomly. Group one (a negative control) was fed the diet without acidifiers, the other groups were fed the diets with 0.1%,0.3% phosphoric acid type acidifier (acidifier A) and 0.1%,0.3%lactic acid type acidifier (acidifier B). Three piglets were randomly selected from each treatment at the end of four week experiment for slaughter test to determine small intestine morphology, large intestinal microbial flora and immune index. The main results showed that: two acidifiers added in the diets significantly increased the length of intestinal villi (P < 0.05); Two acidifiers added in the diets significantly decreased E.coli counts and salmonella counts (P < 0.05), increased lactobacillus counts and bifidobacteria counts (P < 0.05). It seemed that acidifier B was better than acidifier A based on the current measurements; Both of two acidifiers added in the diets significantly increased the content of serum IgA and IgG (P < 0.05). However, the serum IgM content and the ratio of CD4 +/CD8 + were not significantly affected by the types of acidifiers(P > 0.05).