OPS法玻璃化冷冻兔胚胎的研究

采用2种不同浓度的冷冻液(VSⅠ和VSⅡ),通过OPS法玻璃化冷冻兔的胚胎。结果表明:冷冻兔的桑椹胚641枚,解冻后胚胎总回收率为93.8%(601/641),形态结构良好率为94.5%(568/601),透明带完好率为100%(601/601)。VSⅠ和VSⅡ冷冻的胚胎解冻后回收率分别为93.8%(305/325)和93.7%(296/316),两者差异不显著(P>0.05);形态良好率分别为95.1%(290/305)和94.0%(278/296),前者显著地高于后者(P<0.05)。将解冻后形态结构良好的桑椹胚415枚移植到29只受体母兔体内,总妊娠率为51.7%(15/29),产仔率为34.5%(61/177)。VSⅠ妊娠率为53.3%(8/15),产仔率为36.8%(35/95);VSⅡ妊娠率为50.0%(7/14),产仔率为31.7%(26/82)。无论妊娠率还是产仔率,VSⅠ都高于VSⅡ,但差异不显著(P>0.05)。

Study on Vitrification of Rabbit Embryos with the Open Pulled Straw(OPS) Method

Rabbit embryos were vitrified with two different concentration freezing media(VSⅠand VSⅡ)with the OPS method.The results showed(1)641 morulas were vitrified.After thawing,the overall embryos' recovery rate was 93.8%(601/641),568 of which(94.5%)were morphologically intact embryos,and all of 601 embryos' zona pellucid were intact(100%).The embryos' recovery rate of VSⅠand VSⅡwere 93.8%(305/325)and 93.7%(296/316)respectively.290(95.1%)and 278(94.0%)were morphologically intact embryos for VSⅠand VSⅡ,respectively.There was no difference in the embryos' recovery rate between VSⅠ and VSⅡ,and the rate of morphologically intact embryos for VSⅠwas remarkably higher than that for VSⅡ(P < 0.05).(2)After thawing,415 morulas of good quality were transferred into 29 recipients.The overall pregnant rate was 51.7%(15/29)and the rate of embryos developing into young pups was 34.5%(61/177).The pregnant rate and the rate of embryos developing into young pups for VSⅠwere 53.3%(8/15)and 36.8%(35/95),respectively.The correspondent values for VSⅡwere 50.0%(7/14)and 31.7%(26/82).The values of VSⅠwere higher than that of VSⅡ,but the difference was not significant(P > 0.05).