| 猪输卵管上皮细胞、颗粒细胞、耳上皮细胞及胎儿成纤维细胞的培养 |
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| 引用本文： | 吴中红,邢凤英,刘国世,曾申明,张忠诚.猪输卵管上皮细胞、颗粒细胞、耳上皮细胞及胎儿成纤维细胞的培养[J].中国畜牧杂志,2003,39(6):5-6. |
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| 作者姓名： | 吴中红 邢凤英 刘国世 曾申明 张忠诚 |
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| 作者单位： | 中国农业大学动物科技学院,北京,100094 |
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| 基金项目： | 国家自然科学基金 (30 1 0 0 1 33)资助 |
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| 摘 要： | 本文培养了猪输卵管上皮细胞、颗粒细胞、耳上皮细胞、胎儿成纤维细胞等几种细胞 ,比较了几种细胞原代和传代培养的特点 ,发现原代培养时猪耳组织、胎儿组织消化后的单细胞和剩余细胞团块一起培养 ,其生长速度较单个细胞快。 4种细胞中颗粒细胞体积最大 ,生长速度最慢 ,传代密度须保持 2× 10 5 ml以上为宜。
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| 关 键 词： | 畜牧、兽医科学基础学科 体细胞 培养 猪 |
| 文章编号： | 0258-7033(2003)06-0005-02 |
| 修稿时间： | 2002年12月3日 |
The Culture of Porcine Oviduct Epithelium Cells, Cumdus Cells, Ear Epithelium Cells and Fetal Fibroblasts |
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| WU Zhong-hong,Xing Feng-ying,LIU Guo-shi,ZENG Shen-ming,ZHANG Zhong-cheng.The Culture of Porcine Oviduct Epithelium Cells, Cumdus Cells, Ear Epithelium Cells and Fetal Fibroblasts[J].Chinese Journal of Animal Science,2003,39(6):5-6. |
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| Authors: | WU Zhong-hong Xing Feng-ying LIU Guo-shi ZENG Shen-ming ZHANG Zhong-cheng |
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| Abstract: | Porcine oviduct epithelium cells, cumulus cells, ear epithelium cells and fetal fibroblasts were cultured and their characteristics in subculture were studied. The growing speed on cell masses of porcine ear and fetal tissues were faster than those of single cells when single cells and cell masses were cultured together after digestion. Cumulus cells had the biggest diameter and slowest growing speed and a density of at lest 2×10~5 for normal subculture was needed. |
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| Keywords: | Basal disciplines of animal and veterinary science Somatic cells Culture Porcine |
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