小鼠4、8-细胞胚胎单卵裂球体外培养体系的研究

以CZB为基础培养液,培养小鼠4、8-细胞胚胎单卵裂球,研究葡萄糖、牛磺酸和猪输卵管上皮细胞共培养在其体外发育中的作用。结果表明:牛磺酸添加与否对4、8-细胞胚胎单卵裂球的囊胚发育率分别为29%、30%和14%、14%,无显著性差异(P>0.05)。添加葡萄糖后,4、8-细胞胚胎单卵裂球的囊胚发育率分别为36%和19%,有显著提高(P<0.05)。含有葡萄糖而牛磺酸的添加与否对4、8-细胞胚胎单卵裂球的囊胚发育率分别为36%、38%和19%、20%,无显著性差异(P>0.05)。各组内4、8-细胞胚胎单卵裂球形成的囊胚细胞数分别为(10.44±1.24～(12.43±1.18)和(7.57±0.97)～(8.48±1.16),均无显著性差异(P>0.05)。4、8-细胞胚胎单卵裂球与猪输卵管上皮细胞共培养,其囊胚发育率分别为47%和26%,囊胚细胞数分别为(17.57±1.13)和(11.43±0.92),均高于单一培养(P<0.05)。

Study on in vitro Cultural System of Blastomeres Isolated from Mouse 4, 8-cell Embryos

CZB medium was used as a base medium to study the function of taurine, glucose and porcine oviductal epithelial cells system in the culture of blastomeres isolated from mouse 4?8-cell embryos. These results showed addition of taurine in CZB did not significantly affect the blastocyst rate of blastomeres isolated from mouse 4, 8-cell embryos (29% vs 30% and 14% vs 14%) (P > 0.05). Addition of glucose in CZB had a significant effect on the blastocyst rate of blastomeres isolated from mouse 4,8-cell embryos (29% vs 36% and 14% vs 19%) (P < 0.05). Addition of taurine did not have a significant effect on the blastocyst rate of blastomeres isolated from mouse 4?8-cell embryos respectively(36% vs 38% and 19% vs 20%) when glucose had been added in CZB(P > 0.05). The cell number of blastocysts derived from blastomeres isolated from mouse 4, 8-cell embryos were not different significantly(10.44±1.24)～(12.43±1.18) and (7.57±0.97)～(8.48±1.16) respectively (P > 0.05). The blastocyst rate of co-cultured blastomeres isolated from mouse 4, 8-cell embryos were 47% and 26%, respectively; the cell number of blastocysts derived from co-cultured blastomeres isolated from mouse 4, 8-cell embryos were 17.57±1.13 and 11.43±0.92 , repectively. These were more than those of blastomeres in the controls(P < 0.05).