伪狂犬病毒变异株（JS-2012）对仔猪的致病性研究

本实验室2012年从江苏省太仓市某发病猪场分离到1株变异的伪狂犬病毒,命名为JS-2012。为了研究该分离株对仔猪的致病性,将12头15日龄的伪狂犬病毒阴性仔猪随机分为3组,其中2组（每组5头猪）分别通过肌肉注射和滴鼻接种JS-2012毒,第3组2头猪做空白对照。接种病毒的仔猪在攻毒后24 h体温均开始升高,4 d后开始死亡,死亡率为100%。临床观察发现,滴鼻组仔猪发病死亡明显快于肌肉注射组。对病死猪进行剖解,均可见大脑血管扩张并出血等典型的伪狂犬病病理变化。病理切片显示猪的脑蛛网膜下腔严重出血,其他各脏器也均有严重病理变化。结果表明该变异株JS-2012为伪狂犬强毒毒株。

PATHOGENICITY OF VARIANT STRAIN JS-2012 OF PSEUDORABIES VIRUS IN PIGLETS

In December 2012, a variant strain JS-2012 of porcine Pseudorabies virus （PRV） was isolated from a diseased piglet in Taicang city Jiangsu Province. To study the pathogenicity of JS-2012, twelve 15-day-old PRV-free piglets were divided into 3 groups： intramuscular injection group （5 piglets）, intranasal inoculation group （5 piglets） and non-treatment control group （2 piglets）. At 24 h post infection, body temperature of piglets in ten 15-day-old inoculation piglets in two infection groups increased. At day 4 post infection, piglets in both infected group started to die. The intranasal group died faster than those in intramuscular injection group although the mortality of both infection groups was 100%. Dead piglets were necropsied for pathological examination. Vasodilatation and angiorhagia were found in brain, which were typical lesions caused by PRV. Severe subarachnoid hemorrhage and lesions in other organs were also recorded. In conclusion, JS-2012 was a virulent PRV variant.