添加胰岛素对胎儿宫内发育迟缓新生仔猪胰腺生长发育的影响

(目的和方法)为研究添加胰岛素对IUGR新生仔猪胰腺重量、酶活性的影响,试验选择初生IUGR仔猪15头,分为新生对照组(N)、牛乳组(M)和试验组即牛乳添加胰岛素(60 IU/L)组(I),人工饲喂3 d后宰杀取样。(结果)结果显示,与N组相比,饲喂3 d后,M组和I组仔猪胰腺重量、胰腺重/体重、蛋白质含量、DNA含量、RNA含量、胰淀粉酶活性、胰脂肪酶总活性、胰高血糖素总含量均不同程度提高,而胰总蛋白水解酶活性、胰蛋白酶活性、胰糜蛋白酶总活性、胰腺胰岛素总含量均不同程度降低,变化较明显。与M组相比,I组胰腺蛋白质含量、蛋白质/DNA比值分别提高了61.43%、60.54%(P0.01);I组胰总蛋白水解酶活性、胰淀粉酶活性、胰脂肪酶总活性、胰腺胰岛素总含量、胰高血糖素含量分别提高了10.59%、10.40%、9.37%、14.30%、12.30%(P0.05);胰腺重、胰腺重/体重、胰腺DNA含量、RNA含量、RNA/DNA比值也不同程度提高(P0.05);而I组与M组间胰蛋白酶活性、胰糜蛋白酶活性差异不显著。(结论)表明IUGR仔猪生后胰腺生长发育较迅速,添加胰岛素能在一定程度上刺激IUGR仔猪胰腺细胞增殖和增大,能显著促进IUGR仔猪胰腺蛋白质合成和沉积,有刺激胰腺内外分泌部发育、提高胰腺酶活性提高、刺激组织激素分泌的趋势,对IUGR仔猪出生后胰腺的生长具有一定的补偿作用。

Effects of oral insulin on the pancreatic growth and development of IUGR pigs

(Objective and Method) In order to investigate the effects of oral insulin on the growth and development of pancreatic in in-trauterine growth retardation (IUGR) piglets, fifteen Sutai IUGR piglets were selected and divided into three groups:newborn group(N), milk group(M) and milk supplemented with insulin(60 IU/L)group(I). The piglets in group M and I were fed artificially for 3 days, re-spectively.Compared with newborn group, pancreatic tissue weight, the raito tissue weight to body weight, content of protein, content of DNA, content of RNA, total activities of amylase and lipase, content of glucagon in bottled-fed piglets were higher, but total activities of protease, trypsin ,chymotrypsin, and content of insulin were lower . Compared with M group, content of protein, the ratio protein to DNA in pancreas of I group were increased by 61.43%、60.54%(P<0.01), respectively. Compared with M group, total activities of pro-tease, amylase and lipase, and content of Insulin and glucagon in pancreatic of group I were increased by 10.59%、10.40%、9.37%、14.30%、12.30%(P>0.05), respectively. Compared with M group, pancreatic tissue weight, the raito tissue weight to body weight, con-tent of DNA, content of RNA, the ratio RNA to DNA also showed the similar increased tendency (P>0.05). And these difference be-tween total activities of trypsin and chymotrypsin in group M with these in group I didnˊt reach statistical significantly level. (Conclu-sion) The results show that the pancreatic Growth and Development of IUGR piglets were quickly during the early postnatal period, and oral insulin could promote the proliferation and enlargement of pancreatic cells, and could promote synthetic and deposit of pro-tein, and could promote the development of exocrine section and endocrine section in pancreatic to some extent, and could increase activities of protease enzyme, and could promote secretion of hormone, and could promote the pancreatic compensatory growth and de-velopment of IUGR Pigs.