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| 口蹄疫抗体免疫金标快速检测试纸法的建立 | |
| 引用本文： | 张长弓,金颜辉,黄印尧,方莹,梁全顺.口蹄疫抗体免疫金标快速检测试纸法的建立[J].福建畜牧兽医,2004,26(1):4-5. |
| 作者姓名： | 张长弓 金颜辉 黄印尧 方莹 梁全顺 |
| 作者单位： | 1. 厦门大学抗癌研究中心,361005 2. 福建省畜牧兽医总站,360003 3. 厦门出入境检验检疫局,361012 |
| 摘 要： | 应用免疫学原理与胶体金层析技术，采用双抗原夹心ELISA建立了检测口蹄疫(O型)抗体水平的“口蹄疫抗体(O型)免疫胶体金快速检测试纸法”。应用该法与“口蹄疫正向间接血凝抗原法”对300份猪、鸡、鸭、牛、羊等血液或血清进行口蹄疫抗体水平检测试验，符合率达100％。试验结果显示，该法与间接血凝法一样，具有微量、特异、准确等优点，且操作简易，不需要任何仪器，检测时间短，结果直观，容易判定，适用于基层兽医站、养猪场使用和大面积口蹄疫抗体普查。 |
| 关 键 词： | 口蹄疫 抗体检测 免疫金标快速检测试纸法 制备 鉴定 工作原理 |
| 文章编号： | 1003-4331(2004)01-0004-02 |
Study and Apply of Colloidal Gold Strip in Detecting the Antibody of Food and Mouth Disease |
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| Zhang Changgong,Jin Yanhui,Huang Yinyao,Fang Ying,Liang Sunyi.Study and Apply of Colloidal Gold Strip in Detecting the Antibody of Food and Mouth Disease[J].Fujian Journal of Animal Husbandry and Veterinary,2004,26(1):4-5. | |
| Authors: | Zhang Changgong Jin Yanhui Huang Yinyao Fang Ying Liang Sunyi |
| Institution: | Zhang Changgong1,Jin Yanhui2,Huang Yinyao3,Fang Ying1,Liang Sunyi21.Cancer Research Center,Xiamen University,Xiamen,361000,2.3.Xiamen Entry-Exit Inspection and Quarantine Bureau,Xiamen |
| Abstract: | Based on the principle of colloidal gold immunochromatography,a test strip was prepared and applied in detecting the antibody of food and mouth disease(type O).The test result was coincidence with indirect hemagglutination method in examination of 233 blood or sera samples obtained from pig,chicken,duck,cattle and goat.It shows that this strip has the same merits as that of the indirect hemagglutination test in microanalysis,high specialty and accurate.No special instrument was needed and the test can be completed easily in a short time.So it can be used in common animal hospital,hoggery and the general survey of food and mouth disease. |
| Keywords: | Food and mouth disease Colloidal gold |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |