| 半番鸭POU1F1基因序列的克隆与生物信息学分析 |
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| 引用本文： | 朱志明,陈晖,李盛霖,郑嫩珠,缪中纬.半番鸭POU1F1基因序列的克隆与生物信息学分析[J].福建畜牧兽医,2010,32(1):1-4. |
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| 作者姓名： | 朱志明 陈晖 李盛霖 郑嫩珠 缪中纬 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所,福州,350013 |
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| 基金项目： | 福建省农科院青年科技人才创新基金(A2007QJ04)资助 |
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| 摘 要： | 根据GenBank中公布的鸭POU1F1基因序列,设计了6对引物,以半番鸭血液基因组DNA为模板,采用PCR方法扩增出POU1F1基因完整的cDNA序列,全长2209bp。对该序列进行生物信息学分析,结果表明该基因编码335个氨基酸,具有POU-specific(POUs)和Homeobox结构域,与鸡、猪、牛、人和小鼠的POU1F1基因氨基酸序列分别具有96.3%、89.5%、89.2%、90.6%和87.5%的同源性。
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| 关 键 词： | 半番鸭 POU1F1基因 克隆 生物信息学分析 |
Cloning and bioinformatics analysis of POU1F1 gene in mule duck |
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| Zhu Zhiming,Chen Hui,Li Shenglin,Zheng Nenzhu,Miao Zhongwei.Cloning and bioinformatics analysis of POU1F1 gene in mule duck[J].Fujian Journal of Animal Husbandry and Veterinary,2010,32(1):1-4. |
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| Authors: | Zhu Zhiming Chen Hui Li Shenglin Zheng Nenzhu Miao Zhongwei |
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| Institution: | Institute of Animal Husbandry and Veterinary Medicine;Fujian Academy of Agricultural Sciences;Fuzhou 350013 |
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| Abstract: | According to the published sequences of the duck POU1F1 gene, six pairs of specific primers were designed. The cDNA was amplified from the genomic DNA abstracted from mule duck blood by PCR technique. Bioinformatics analysis showed that the gene encode 335 amino acid residues, with conserved POU-specific （POUs） domain and Homeobox domain. The result of identification and analysis indicated that mule duck POU1F1 cDNA shared 96.3% ,89.5% ,89.2% ,90.6% and 87.5% similarity in amino acid sequences with chick, pig, cattle, human and mouse respectively. Key words mule duck POU1F1 cloning bioinformatics analysis |
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| Keywords: | mule duck POU1F1 cloning bioinformatics analysis |
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