材料处理与切片方法对柑橘叶脉荧光显微观察的影响

植物组织结构与细胞形态学观察是植物系统分类、遗传演化、抗逆反应等的主要研究方法之一。为了简便、有效的进行植物组织细胞的显微观察，本文研究了不同材料处理和不同切片方法对柑橘叶脉组织细胞荧光显微观察的影响。结果表明：利用荧光显微技术可以简便、有效的观测柑橘叶脉的组织结构和细胞形态；新鲜样品徒手切片和冷冻切片、FAA固定样品冷冻切片在荧光显微镜下都可以得到较佳的观测效果；几种样品处理方法各有优缺点，研究中可以根据具体情况灵活选用。

Effects of material treatments and slicing methods on fluorescence microscopic observation of citrus leaf vein

The microscopic observation of plant tissue and cell is one of the main research methods to study on plant taxonomy, genetic evolution and stress resistance. In order to find a simple and effective microscopic observation method, the fluorescence microscopic technique was applied. The effects of different material treatments and slicing methods on fluorescence microscopic observation of citrus leaf vein was studied. The results showed that fluorescence microscopic technology was simple and effective. A better effect of fluorescence microscopic observation was got by using fresh sample with free-hand or frozen slicing and fixed sample(FAA) with frozen slicing. Each method has its own advantages and disadvantages.