| AFLP分析人工老化对扁蓿豆遗传完整性的影响 |
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| 引用本文： | 宿宇,王建光,卢新雄.AFLP分析人工老化对扁蓿豆遗传完整性的影响[J].草地学报,2012,20(1):125-129. |
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| 作者姓名： | 宿宇 王建光 卢新雄 |
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| 作者单位： | 1. 内蒙古农业大学生态环境学院,内蒙古呼和浩特,010018
2. 中国农业科学院作物科学研究所,北京 100081;农业部作物种质资源与生物技术重点开放实验室,北京 100081 |
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| 基金项目： | 农业部农作物种质资源保护利用项目 |
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| 摘 要： | 采用AFLP分子标记对经过人工老化处理的不同发芽率水平的扁蓿豆(Trigonella ruthenica L.)种质(00-408)进行分析,以探索人工老化在分子水平上对扁蓿豆种质遗传完整性的影响。结果表明:老化处理群体的多态位点百分比、有效等位基因数、遗传多样性指数和Shannon指数均低于对照群体(P>0.05)。因此,人工老化处理使扁蓿豆种质的遗传多样性水平有所下降,但对其遗传完整性没有显著影响,由此表明扁蓿豆种质的耐贮性较强。
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| 关 键 词： | 扁蓿豆 人工老化 AFLP 遗传完整性 |
Influence of Ageing on Genetic Integrity of Trigonella ruthenica L. |
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| SU Yu
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WANG Jian-guang
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LU Xin-xiong.Influence of Ageing on Genetic Integrity of Trigonella ruthenica L.[J].Acta Agrestia Sinica,2012,20(1):125-129. |
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| Authors: | SU Yu WANG Jian-guang LU Xin-xiong |
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| Institution: | 2,3(1.College of Ecology and Environment Science,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018,China; 2.Crop Science Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China; 3.Key Laboratory of Crop Germplasm and Biotechnology,Ministry of Agriculture,Beijing 100081,China) |
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| Abstract: | Artificially aged Trigonella ruthenica L.germplasm 00-408 with different germination rates was analyzed by AFLP molecular markers to explore the influence of artificial aging on the genetic integrity of Trigonella ruthenica L.germplasm at molecular levels.Results showed that the percentage of polymorphic loci(PPB),effective number of alleles(Ne),Nei’s gene diversity index(H),Shannon’s information index(I) of aging treatments were lower than that of control,but there was no significant difference base on analysis of variance.This indicated that artificial aging caused declining genetic diversity but did not significantly affect the genetic integrity of Trigonella ruthenica L.germplasm.Therefore,Trigonella ruthenica L.germplasm has a high storability. |
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| Keywords: | Trigonella ruthenica L Artificial aging AFLP Genetic integrity |
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