碱茅种子发芽检测标准方法的研究

于2008年以碱茅(Puccinellia distans Parl)种子为材料,研究在10-25℃、10-30℃、15-25℃、15-30℃、20-30℃变温处理和15℃、20℃、25℃、30℃恒温以及预处理条件下,筛选确定碱茅种子发芽的最适温度和光照条件条件,为碱茅种子质量检测的标准化提供参考依据。结果表明:碱茅种子在20-30℃8 h高温光照16 h低温黑暗条件下发芽率最高,预处理对碱茅种子发芽无显著影响。碱茅种子发芽计数时间确定为第7 d进行初次计数,第14 d进行末次计数。并在此温度条件下选用5个碱茅种子样品进行发芽测定,可以反映碱茅种子的发芽水平,而且硝酸钾和预先冷冻处理对碱茅种子发芽促进作用不明显。

Study on the Standard Methods of Germination Testing in Puccinellia distans Seeds

The experiments were designed to provide standard methods of germination testing for Puccinellia distans seeds and determine the suitable germination conditions in alternative temperatures (10-25℃, 10-30℃, 15-25℃, 15-30℃, 20-30℃), consistent temperatures (15℃, 20℃, 25℃, 30℃) and pretreatments . The results showed that the highest germination rate of Puccinellia distans seeds was at the condition of alternative temperature 20-30℃ and 8 h light compared with 16 h darkness, some pretreatments including prechill, KNO_3 and GA didn't promote seed dormancy. The first count time was 7~(th) day and the final count time was 14~(th) day based on the seed samples lot2003 and lot2004 tested. In addition, five seed samples were germinated and validated the suitable condition, the results could show the potential of seed germination, and seed dormancy wasn't broken under prechill and KNO3 pretreatments.