独脚金内酯对干旱胁迫下黑果枸杞种子萌发和幼苗生理变化的影响

本试验以黑果枸杞(Lycium ruthenicum)为研究对象，探讨其种子萌发和幼苗生理特性对干旱胁迫下外源独脚金内酯(Strigolactones, SLs)的响应。试验通过聚乙二醇(Polyethylene glycol, PEG-6000)模拟干旱胁迫，对黑果枸杞种子和幼苗进行不同浓度SLs处理，测定各处理对种子发芽率、发芽势、发芽指数、活力指数及幼苗叶绿素、脯氨酸(Proline, Pro)、丙二醛(Malondialdehyde, MDA)含量和总抗氧化能力(Total antioxidant capacity, T-AOC)的影响。结果表明：SLs浸种对干旱胁迫下黑果枸杞种子萌发具有低浓度促进、高浓度抑制效果；干旱胁迫下对幼苗喷施适宜浓度SLs,可显著提高其叶绿素、Pro含量(P<0.05),同时有增强T-AOC、降低MDA积累的作用；主成分分析显示1μmol·L^-1 SLs处理能够增强黑果枸杞的抗旱能力。综上所述，SLs不仅可以缓解干旱胁迫对黑果枸杞种子萌发的抑制，还能增强幼苗抗旱性，最佳使用浓度为1μmol·L^-1。本...

Effects of Strigolactones on Seed Germination and Seedling Physiological Changes of Lycium ruthenicum under Drought Stress

This study investigated the effects of exogenous strigolactones (SLs) on seed germination and seedling physiological characteristics of Lycium ruthenicum under drought stress. The seeds and seedlings of Lycium ruthenicum were treated with different concentrations of SLs and used PEG-6000 to simulate drought stress. The effects of each treatment on seed germination rate,germination potential,germination index,vigor index,and the contents of chlorophyll,proline (Pro),malondialdehyde (MDA),and total antioxidant capacity (T-AOC) of seedlings were determined. The results showed that applying exogenous SLs promoted seed germination of Lycium ruthenicum at low concentrations and inhibit seed germination at high concentrations under drought stress. Spraying SLs with appropriate concentration could significantly increase the contents of chlorophyll and Pro (P<0.05),enhance T-AOC,and decrease MDA accumulation under drought stress. Principal component analysis showed that 1 μmol·L^-1 SLs treatment could enhance the drought resistance of Lycium ruthenicum. In conclusion,SLs could not only alleviate the inhibition of drought stress on seed germination of Lycium ruthenicum,but also enhance the drought resistance of seedlings,and the optimal concentration of SLs is 1 μmol·L^-1. This study provides certain theoretical support for the application of SLs in the artificial cultivation of Lycium ruthenicum.