| 转基因冰草植株耐盐性研究 |
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| 引用本文： | 徐春波,米福贵,王勇. 转基因冰草植株耐盐性研究[J]. 草地学报, 2006, 14(1): 20-23. DOI: 10.11733/j.issn.1007-0435.2006.01.005 |
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| 作者姓名： | 徐春波 米福贵 王勇 |
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| 作者单位： | 1. 中国农业科学院草原研究所, 呼和浩特, 010010;2. 内蒙古农业大学, 呼和浩特, 010019 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 以转P5CSF129A基因的冰草属(Agropyron Gaertn)阳性植株(97#、19#、66#)和阴性植株为材料,测定叶片脯氨酸含量、相对含水量、质膜透性和K+/Na+比值4项耐盐指标。结果表明:转基因植株的脯氨酸含量明显高于阴性植株,说明P5CSF129A基因已经在冰草转化植株体内表达,提高了转基因植株的耐盐性;其它耐盐指标的结果也证明转基因冰草植株的耐盐性高于对照植株。对转基因植株和阴性植株的耐盐能力综合评定结果是97#>19#>66#>阴性植株。
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| 关 键 词： | 冰草 耐盐指标 P5CSF129A基因 |
| 文章编号： | 1007-0435（2006）01-0020-04 |
| 收稿时间： | 2005-03-15 |
| 修稿时间： | 2005-09-23 |
Research on Salt-Tolerance of Wheatgrass Transformed by P5CS Gene |
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| XU Chun-bo,MI Fu-gui,WANG-Yong. Research on Salt-Tolerance of Wheatgrass Transformed by P5CS Gene[J]. Acta Agrestia Sinica, 2006, 14(1): 20-23. DOI: 10.11733/j.issn.1007-0435.2006.01.005 |
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| Authors: | XU Chun-bo MI Fu-gui WANG-Yong |
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| Affiliation: | 1. Grassland Research Institute of Chinese Academy of Agriculture Sciences, Huhhot, Inner Mongolia Autonomous Region 010010, China;2. Inner Mongolia Agriculture University, Huhhot, Inner Mongolia Autonomous Region 010019, China |
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| Abstract: | Plants transformed by P5CS gene(97#、19#、66#) and negatives were tested using several salt tolerance index such as proline content,leaf-water content,plasma membrane permeability and ratio of K+/Na+.The results show that the transgenic wheatgrass plants accumulated more proline than CK under salt-stress conditions,which proved that P5CSF129A had functioned in transgenic wheatgrass plants and had increased their salt-tolerance.The results also show that transgenic wheatgrass plants had nurtured greater salinity tolerance than CK.According to physiological target in synthetically evaluation,the result is 97#>19#>66#>Negatives. |
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| Keywords: | Wheatgrass Salt tolerance P5CSF129A gene |
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