复合微生物对四环素胁迫下多花黑麦草苗期生长和生理特性的影响

为探究复合微生物菌剂对非生物胁迫下多花黑麦草(Lolium multiflorum)的作用效果，本试验以不同浓度(0，50，100，150mg·L^-1)四环素胁迫下多花黑麦草为研究材料，探讨四环素胁迫下添加复合微生物菌剂(MI)对多花黑麦草生长和生理特性的影响。结果表明：添加菌剂(MI)能够增加四环素胁迫下多花黑麦草幼苗根长芽长，增加叶绿素含量、株高根长及地上地下生物量，改善根尖细胞数量及形态，改善根系性状，增加抗氧化酶(SOD,POD及CAT)活性，降低细胞相对电导率及丙二醛(MDA)含量。因此，添加复合微生物强化了多花黑麦草对四环素的降解作用，菌剂对四环素胁迫下多花黑麦草的生长发育具有促进作用，并对四环素胁迫造成的细胞损伤具有修复作用，菌剂的添加提高了多花黑麦草的抗四环素胁迫能力。

Effect of Complex Microorganisms on the Growth and Physiological Characteristics of Lolium multiflorum Seedlings under Tetracycline Stress

In order to investigate the effect of complex microbial agents on Lolium multiflorum under abiotic stress,Lolium multiflorum was subjected to different concentrations (0,50,100,150 mg·L^-1) of tetracycline stress as the study material to investigate the effect of the addition of complex microbial agents (MI) on the growth and physiological characteristics of Lolium multiflorum under tetracycline stress. The results showed that the effect of MI addition on the growth and physiological characteristics of Lolium multiflorum was not significant. The results showed that the addition of MI increased the root and shoot lengths,increased chlorophyll content,root height,and above-ground biomass,improved root tip cell number and morphology,improved root traits,increased antioxidant enzymes (SOD,POD,and CAT) activities,and decreased relative cell conductivity and malondialdehyde (MDA) content of Lolium multiflorum seedlings under tetracycline stress. The results of the above study showed that the addition of complex microorganisms enhanced the degradation of tetracycline in Lolium multiflorum,and the bacteriophage promoted the growth and development of Lolium multiflorum under tetracycline stress and repaired the cell damage caused by tetracycline stress,and the addition of bacteriophage improved the resistance of Lolium multiflorum to tetracycline stress.