猪O型口蹄疫免疫抗体检测方法的比较

试验采用口蹄疫O型间接血凝试验和O型口蹄疫抗体液相阻断ELISA 2种试验方法检测了60份血清中猪O型口蹄疫免疫抗体.研究结果显示,60份被检血清口蹄疫O型间接血凝试验检测合格率为93.3％,O型口蹄疫抗体液相阻断ELISA试验检测的合格率为73.3％,口蹄疫O型间接血凝试验检测合格率明显高于O型口蹄疫抗体液相阻断ELISA试验检测的合格率（相差20个百分点）;2种方法的总符合率为66.7％、＜25（＜2^6）的符合率为28.6％,≥2^5（≥2^6）的符合率为82.1％.2种方法检测出的整体免疫效果较好,平均合格率远高于农业部规定的70％.

Comparison of the Detection Methods of Immune Antibody of O-type Foot-and-mouth Disease of Pigs

The immune antibody of O-type foot-and-mouth disease in 60 serum samples were detected by using two test methods of O-type foot-and-mouth disease indirect hemagglutination antibody and O-type foot-and-mouth disease antibody liquid-phase blocking ELISA. The results showed that the qualification rate of 60 serum samples in O-type foot-and-mouth disease indirect hemagglutination assay was 93.3% and that in O-type foot-and-mouth disease antibody liquid-phase blocking ELISA was 73.3%. The qualification rate in O-type foot-and-mouth disease indirect hemagglutination assay was obviously higher than that in O-type foot-and-mouth disease antibody liquid-phase blocking ELISA （higher 20% ）. The whole coincidence rate of two methods was 66.7%, that of less than 25（less than 26） was 28.6% and that of ≥25（ ≥26）was 82.1%. The whole immune effects of two detection methods were both better, and the average qualification rate was greatly higher than the specified value （70%） of Ministry of Agriculture.