碘乙酰胺和罗丹明对苜蓿原生质体失活效果的效应

以清水紫花苜蓿(Medicago sativa cv.Qingshui))和甘农4号紫花苜蓿(M.sativacv.Gan-nong No.4)愈伤组织原生质体为材料,研究了碘乙酰胺(IOA)和罗丹明6G(R-6G)在5个不同浓度梯度处理下对原生质体细胞质失活效果的影响。结果表明,3～10mmol/L的IOA和4 070μg/mL的R-6G分别处理10min,可使2个品种原生质体的活力及植板率明显下降,品种间差异显著(P<0.05),各处理浓度与活力和植板率之间均存在极显著的负相关(P<0.01)。培养第7d,所有处理均可见再生的小细胞团,培养30～40d,所有高于最低失活浓度的处理原生质体均丧失了再生愈伤组织的能力。适宜2个品种的最佳抑制剂及最低失活浓度分别为3mmol/L IOA或40μg/mL R-6G。

Effects of IOA and R-6G on cytoplasm Inactivation of callus protoplasts of two alfalfa varieties

Cytoplasm inactivation of protoplasts cy by using two alfalfa varieties （Medicago sativa were studied by measuring their viability and plate efficiency. Qingshui,M. sativa cv. Gannong No. 4）. The results showed that viability and plate efficiency of protoplasts of two varieties obviously reduce treated at 3-10 mmol/L IOA and 40-70 alfalfa varieties. The viab ilit concentration of IOA and R / Y 6 mL R-6G with 10 min,and there was significant different （P〈0.05）between two and plate efficiency of two varieties significantly and positively correlated with the G. Regenerative small aggregates could be seen in all treatments in 7 day. Devel opment of protoplasts stopped and cannot form regenerative calli while concentration of IOA and R-6G were higher than lowest inactivation concentration when culture 30 to 40 days. The optimal inhibitor and lowest inac tivation concentrations was 3 mmol/L IOA and 40 μg/mL R-6G respectively.