| 不同培养方式和pH对草地早熟禾原生质体分裂和生长的影响 |
| |
| 引用本文： | 白生军,马晖玲,马祥,安惠惠.不同培养方式和pH对草地早熟禾原生质体分裂和生长的影响[J].草原与草坪,2012,32(1):50-53. |
| |
| 作者姓名： | 白生军 马晖玲 马祥 安惠惠 |
| |
| 作者单位： | ：甘肃农业大学草业学院／草业生态系统教育部重点实验室／甘肃省草业工程实验室／中-美草地畜牧业可持续发展研究中心,甘肃兰州,730070 |
| |
| 摘 要： | 以3个草地早熟禾品种午夜Ⅱ号、新格莱德和橄榄球Ⅱ号继代培养20次的愈伤组织为材料,设置不同的pH水平,通过2种培养方式进行了原生质体培养,观测其原生质体的分裂和生长状况。结果表明:3个草地早熟禾品种原生质体最适宜的培养方式为固液双层培养,固液双层培养中液相pH为5.8(新格莱德pH6.0)时原生质体分裂率达最高值,午夜2号42.8%、新格莱德50.1%和橄榄球2号29.4%。基因型的差异对于原生质体培养成功与否有着密切的关系。
|
| 关 键 词： | 液体浅层培养 固液双层培养 原生质体分裂率 pH |
Effect of different cultivation methods and pH on division and growth of Poa pratensis protoplast |
| |
| BAI Sheng-jun,MA Hui-ling,MA Xiang,AN Hui-hui.Effect of different cultivation methods and pH on division and growth of Poa pratensis protoplast[J].Grassland and Turf,2012,32(1):50-53. |
| |
| Authors: | BAI Sheng-jun MA Hui-ling MA Xiang AN Hui-hui |
| |
| Institution: | (College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S. Centers for Grazingland Ecosystem Sustainability Lanzhou 730070,China) |
| |
| Abstract: | The callus sub-cultured for 20 times from 3 varieties of Poa pratensis(MidnightⅡ,Nuglade and RugbyⅡ)used for protoplast culture through different cultivation methods and pH values.The result indicated that solid-liquid double layer was better for protoplast culture.The peaks of protoplast division rates were 42.8% for Midnight Ⅱ(pH 5.8),50.1% for Nuglade(pH 6.0) and 29.4% for RugbyⅡ is 29.4%(pH 5.8) respectively.The genotype difference showed an important effect on protoplast culture. |
| |
| Keywords: | liquid shallowculture solid-liquid double layer culture protoplast division rate pH |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
