| 植物激素对2个紫花苜蓿品种再生体系的影响 |
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| 引用本文： | 王珺,柳小妮.植物激素对2个紫花苜蓿品种再生体系的影响[J].草原与草坪,2010,30(6):15-18. |
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| 作者姓名： | 王珺 柳小妮 |
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| 作者单位： | 甘肃农业大学,草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃,兰州,730070 |
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| 基金项目： | 国家科技支撑计划“优质高产抗逆苜蓿和饲料作物新品种选育(2006BAD04A04-01)”项目资助 |
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| 摘 要： | 采用MS为基本培养基,附加不同浓度的2,4-D、KT和NAA,对"盛世"和"甘农4号"2个紫花苜蓿品种进行了组织培养再生体系的研究。结果表明:下胚轴是愈伤组织诱导的最佳外植体材料;2,4-D浓度增加对"甘农4号"紫花苜蓿出愈率影响不明显,但"盛世"紫花苜蓿的出愈率随着2,4-D浓度的增加呈显著升高趋势;2个品种的最佳愈伤组织诱导培养基为MS+2 mg/L 2,4-D+0.3 mg/L KT+30g/L蔗糖+7.5 g/L琼脂;最适分化培养基为MS+KT0.5 mg/L+NAA0.1 mg/L+蔗糖20 g/L;最佳诱导生根的培养基为1/2 MS+NAA0.5 mg/L。
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| 关 键 词： | 紫花苜蓿 愈伤组织 再生体系 植物激素 |
Effect of phytohormone on regeneration system of two alfalfa varieties |
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| WANG Jun,LIU Xiao-ni.Effect of phytohormone on regeneration system of two alfalfa varieties[J].Grassland and Turf,2010,30(6):15-18. |
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| Authors: | WANG Jun LIU Xiao-ni |
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| Institution: | WANG Jun,LIU Xiao-ni (College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Grassl, Ecosystem,Ministry of Education,Sino-U.S.Center for Grazingl, Ecosystem Sustainability,Lanzhou 730070,China) |
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| Abstract: | The regenerative systems of alfalfa varieties(Millennium and Gannon No.4)were comparatively studied by using MS as basic medium adding different concentration of 2,4-D,KT and NAA.The results showed that the hypocotyl was the optimal explant material;2,4-D level had the less effect to callus induction of Gannon No.4,but evidently enhanced Millennium with the increasing concentration of 2,4-D.The effective media for inducing callus of these two variety was MS containing 2 mg/L 2, 4-D ,0.3 mg/L KT,30 g/L sucro... |
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| Keywords: | Medicago sativa embryogenic callus regeneration system phytohormone |
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