| 苜蓿花药愈伤组织胚状体诱导因素的研究 |
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| 引用本文： | 耿小丽,魏臻武,姚喜红.苜蓿花药愈伤组织胚状体诱导因素的研究[J].草原与草坪,2011,31(3):77-80. |
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| 作者姓名： | 耿小丽 魏臻武 姚喜红 |
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| 作者单位： | 1. 甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心/甘肃省草业工程实验室,甘肃兰州,730070
2. 扬州大学动物科学与技术学院,江苏扬州,225009 |
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| 基金项目： | 国家自然科学基金(30671486); 国家863计划项目(2008AA10Z149)资助 |
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| 摘 要： | 以7个基因型的苜蓿花药愈伤组织为材料,进行了基因型、愈伤组织生长率、培养温度、培养基碳源等因素对苜蓿花药培养诱导胚状体影响的研究.结果表明:不同基因型苜蓿花药愈伤组织生长率、胚状体诱导率都存在显著性差异,两者的相关系数r=-0.326 1(P＜0.01),两者没有明显的相关性;低温处理可促进花药愈伤组织的分化,4℃低温...
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| 关 键 词： | 苜蓿 花药培养 胚状体 诱导率 |
Study on the introduction factors of callus embryoid of Medicago sativa anther |
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| GENG Xiao-li,WEI Zhen-wu,YAO Xi-hong.Study on the introduction factors of callus embryoid of Medicago sativa anther[J].Grassland and Turf,2011,31(3):77-80. |
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| Authors: | GENG Xiao-li WEI Zhen-wu YAO Xi-hong |
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| Institution: | GENG Xiao-li1,WEI Zhen-wu2,YAO Xi-hong1(1.College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/Sino-U.S.Centers for Grazingland Ecosystem Sustainability/Pratacultural Engineering Laboratory of Gansu Province,Lanzhou 730070,China,2.College of Animal Science & Technique,Yangzhou University,Yangzhou,225009,China) |
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| Abstract: | Seven genotypes of callus of alfalfa anther were used to study the effects of genotype,growth rate of callus,temperature and carbon source of culture on introduction of embryoid of alfalfa anther.The results showed that different genotypes were significant factors to growth rate of anther callus and induction rate of embryoid.Low temperature of culture can improve the differentiation rate of anther callus.The treatment of 4 ℃ with the precondition of 48 hours was best approach.The carbon source of culture also influenced on the embryoid induction,and the effect of culture with sucrose was better than other cultures with lactose and glucose. |
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| Keywords: | Medicago sativa anther culture embryoid inductive rate |
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