| 结缕草根际联合固氮菌培养条件研究 |
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| 引用本文： | 张晓波,赵艳.结缕草根际联合固氮菌培养条件研究[J].草原与草坪,2011(5):65-68. |
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| 作者姓名： | 张晓波 赵艳 |
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| 作者单位： | 海南大学农学院/热带作物种质资源保护与开发利用教育部重点实验室;山西农业大学资源环境学院 |
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| 基金项目： | 海南省自然科学基金(309009)“结缕草根际联合固氮菌的分离、鉴定及筛选”资助 |
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| 摘 要： | 以结缕草根际土壤中筛选得到的5株联合固氮菌为试验材料,对其培养条件以及典型生长曲线开展研究。结果表明,菌株在10～45℃均可生长,最适生长温度在20～30℃;最适生长初始pH值在7.0～7.5;菌株N4、N5、N6、N8的最佳通气量为220mL,菌株N1最佳通气量为200mL;并在上述基础上经过进一步试验得到了菌株的典...
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| 关 键 词： | 结缕草 联合固氮菌 培养条件 典型生长曲线 |
Culture condition of associative nitrogen-fixing bacteria isolated from rhizosphere of Zoysia japonica |
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| ZHANG Xiao-bo,ZHAO Yan.Culture condition of associative nitrogen-fixing bacteria isolated from rhizosphere of Zoysia japonica[J].Grassland and Turf,2011(5):65-68. |
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| Authors: | ZHANG Xiao-bo ZHAO Yan |
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| Institution: | 1.College of Agriculture,Hainan University,Hainan Danzou 571737;2.Key Laboratory of Protection and Development Utilization of Tropical Grop Germplasm Resources(Hainan University),Ministry of Education;3.College of Resources & Environment,Shanxi agricultural university,Shanxi Taigu 030801) |
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| Abstract: | Culture conditions and typical growth curve of five associative nitrogen-fixing bacteria strains,isolated from rhizosphere of Zoysia japonica,were studied.The results showed that all the tested strains could grow under 10 ℃ to 45 ℃,the optimum temperature for strains growth was from 20 ℃ to 30 ℃,and the optimum initial pH range was 7.0 to 7.5,the optimum ventilation volume was 220 mL for strain N4,N5,N0 and N8,while 200 mL for N1.And the typical growth curve of strains obtained as well in the present paper. |
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| Keywords: | Zoysia japonica associative nitrogen-fixing bacteria culture condition typical growth curve |
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