| 硫酸铜,硝酸银和脱落酸对草地早熟禾胚性愈伤诱导的影响 |
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| 引用本文： | 罗莉,韩烈保,高彩霞,Klaus K Nielsen.硫酸铜,硝酸银和脱落酸对草地早熟禾胚性愈伤诱导的影响[J].草原与草坪,2005(4):20-24. |
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| 作者姓名： | 罗莉 韩烈保 高彩霞 Klaus K Nielsen |
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| 作者单位： | 北京林业大学草坪研究所,北京,100083;DLF Trifolium A/S,Store Heddinge,Denmark,4660 |
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| 基金项目： | 丹麦DLF-Trifolium公司资助项目 |
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| 摘 要： | 试验以草地早熟禾4个品种的成熟种子为外植体,研究了CuSO4、AgNO3、脱落酸(ABA)对草地早熟禾胚性愈伤诱导的影响,结果表明,K3诱导培养基中添加CuSO4对草地早熟禾胚性愈伤组织的形成有促进作用,除DP192-10之外的3个品种都发生了明显的形态上的变化,DP-LF-300和2W42-117在添加5μmol/LCuSO4的培养基上诱导出了乳白色、致密的胚性愈伤组织,2W42-117的胚性愈伤率高达40%。在MS基本培养基中添加AgNO3、ABA对草地早熟禾4个品种的胚性愈伤组织的诱导没有作用。
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| 关 键 词： | 草地早熟禾 胚性愈伤组织 AgNO3 ABA CuSO4 |
| 文章编号： | 1009-5500（2005）04-0020-05 |
| 收稿时间： | 2005-03-04 |
| 修稿时间： | 2005年3月4日 |
Effect of CuSO4, AgNO3 and ABA on inducing of embryogenic callus of Kentucky Bluegrass |
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| LUO Li,HAN Lie-bao,Gao Cai-xia,Klaus K Nielsen.Effect of CuSO4, AgNO3 and ABA on inducing of embryogenic callus of Kentucky Bluegrass[J].Grassland and Turf,2005(4):20-24. |
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| Authors: | LUO Li HAN Lie-bao Gao Cai-xia Klaus K Nielsen |
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| Abstract: | The effect of CuSO_4,AgNO_3and ABA on embryogenic callus inducing to 4 varieties of Kentucky bluegrass (Poa pratensis L.) was investigated based on explant derived from mature seeds. The results showed that K3 induction medium supplemented with CuSO_(4 )improved the formation of embryogenic callus. DP-LF-300 and 2W42-117 produced white to yellow, compact and friable embryogenic callus on K3 medium supplemented with 5 mol/L CuSO_(4). High efficiency of embryogenic callus was obtained up to 40% in 2W42-117. While additionally supplemented AgNO_(3)and ABA in MS based medium showed ineffective on embryogenic callus formation. |
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| Keywords: | AgNO3 ABA CuSO4 |
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