| 6株溶磷菌和4株固氮菌混合培养条件的研究 |
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| 引用本文： | 刘青海,姚拓,马从,李显刚.6株溶磷菌和4株固氮菌混合培养条件的研究[J].草原与草坪,2011,31(6):1-6,13. |
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| 作者姓名： | 刘青海 姚拓 马从 李显刚 |
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| 作者单位： | 甘肃农业大学草业学院／草业生态系统教育部重点实验室／甘肃省草业工程实验室／中一美草地畜牧业可持续发展研究中心,甘肃兰州,730070 |
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| 基金项目： | 国家自然基金“苜蓿根际溶磷菌与根瘤菌互作及促生机理研究”(30960265)资助 |
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| 摘 要： | 对6株溶磷菌和4株固氮菌混合培养，选择最适培养条件即温度，pH和盐分，为确定促生菌的最佳发酵条件和生态适应性评价提供依据。结果表明：溶磷菌和固氮菌单独培养或混合培养最适温度在25-35℃，混合培养较单独培养生长良好；溶磷菌和固氮菌单独培养或混合培养最适pH为6．5～7．5，在pH7．5～8．5偏碱性环境下，混合培养生长...
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| 关 键 词： | 溶磷菌 固氮菌 混合培养 温度 pH 盐分 |
Cultural conditions for mixed culture of phosphate solubilizing and nitrogen-fixing bacteria |
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| Institution: | LIU Qing-hai,YAO Tuo,MA Cong,LI Xiang-gang(College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province/Sino-U.S. Centers for Grazingland Ecosystem Sustainability Lanzhou 730070,China) |
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| Abstract: | The cultural conditions(temperature,pH and salinity) for phosphate solubilizing bacteria and nitrogen-fixing bacteria was determined and analyzed in order to provide a basis for the optimum fermentation conditions and evaluation of ecological adaptation.The results showed that the optimum temperature for separate or mixed culture of phosphate solubilizing bacteria and nitrogen-fixing bacteria was ranged from 25 to 35 ℃,former culture was better than the latter.The optimum pH was ranged from 6.5 to 7.5.When pH was 7.5 to 8.5,the mixed culture was better compared to separate culture.The bacteria combinations of Lx81+W5,Lx191+W5 and Jm92+W5 Cou′d grew in 7% of the salt concentration,which performed the stronger salt tolerant compared to separate culture. |
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| Keywords: | phosphate solubilizing bacteria nitrogen-fixing bacteria mixed culture temperature pH salt |
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