| 热应激绍兴鸭不同组织HSP70基因mRNA表达研究 |
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| 引用本文： | 王德前,田勇,李进军,沈军达,陶争荣,李国勤,卢立志.热应激绍兴鸭不同组织HSP70基因mRNA表达研究[J].中国家禽,2012,34(6):24-28. |
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| 作者姓名： | 王德前 田勇 李进军 沈军达 陶争荣 李国勤 卢立志 |
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| 作者单位： | 浙江省农业科学院畜牧兽医研究所,浙江杭州,310021 |
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| 基金项目： | 浙江省优先主题重点国际科技合作研究项目(2010C14014);水禽产业技术体系—蛋鸭育种科学家岗位(CARS-43-2) |
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| 摘 要： | 研究蛋鸭热应激条件下不同组织中热休克蛋白70(HSP70)基因mRNA表达规律,为揭示蛋鸭热应激反应机理提供参考。60只绍兴蛋鸭25℃饲养20d,其中30只进行40℃热应激处理1h,采取荧光定量PCR方法分别测定不同组织中HSP70mRNA的表达水平。试验组与对照组相比,肝脏、脾脏和胰腺组织HSP70mRNA表达差异不显著,其他6种组织HSP70基因表达量均有不同程度的增加,试验组心脏、腿肌、垂体、下丘脑、胸肌、肾脏中HSP70基因表达量分别高于对照组3.6倍、2.8倍、2.7倍、2.4倍、2.2倍和1.57倍。
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| 关 键 词： | 热休克蛋白70(HSP70) 热应激 基因表达 |
Study on HSP70 mRNA Expression of Different Tissues in Shaoxing Duck under Heat Stress |
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| WANG Deqian
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TIAN Yong
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LI Jinjun
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SHEN Junda
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TAO Zhengrong
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LI Guoqin
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LU Lizhi.Study on HSP70 mRNA Expression of Different Tissues in Shaoxing Duck under Heat Stress[J].China Poultry,2012,34(6):24-28. |
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| Authors: | WANG Deqian TIAN Yong LI Jinjun SHEN Junda TAO Zhengrong LI Guoqin LU Lizhi |
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| Institution: | (Institute of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021) |
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| Abstract: | This study was to investigate the expression profiles of heat shock protein 70(HSP70) mRNA in different tissues of laying ducks under heat stress,and provide reference for exploring the mechanism of heat stress reaction.60 Shaoxing ducks were raised at the temperature of 25 ℃ for 20 days,among which 30 ducks were treated by heat stress of 40 ℃ for 1 hour.Expression levels of HSP70 mRNA in tissues involved were measured using fluorescence quantitative PCR.The results showed that gene expression of liver,spleen and pancreas in experimental group were scarcely higher,meanwhile,expression levels of HSP70 mRNA in other 6 tissues were 3.6 times in heart,2.8 times in leg muscle,2.7 times in pituitarium,2.4 times in hypothalamus,2.2 times in breast muscles and 1.57 times in kidney higher than that of the control. |
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| Keywords: | heat shock protein 70 heat stress gene expression |
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