| 高效液相-串联质谱法测定鸡肉中泰妙菌素残留的研究 |
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| 引用本文： | 耿士伟,朱志谦,曲斌,蒋天梅,陆桂萍.高效液相-串联质谱法测定鸡肉中泰妙菌素残留的研究[J].中国家禽,2012,34(11):28-31. |
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| 作者姓名： | 耿士伟 朱志谦 曲斌 蒋天梅 陆桂萍 |
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| 作者单位： | 江苏省畜产品质量检验测试中心,江苏南京,210036 |
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| 摘 要： | 建立了固相萃取-高效液相色谱-串联质谱法测定鸡肉中泰妙菌素残留的分析方法。试样经磷酸盐缓冲液(pH8.0)-乙酸乙酯混合提取,经水饱和正己烷除脂后,过MCX固相萃取小柱净化,采用HPLC-MS/MS电喷雾电离正离子扫描,多反应监测模式(MRM)检测,内标法定量。泰妙菌素在0.5~50μg/L的浓度范围内呈现良好的线性关系,相关系数R2大于0.999。其检测限为0.25μg/kg,定量限为0.5μg/kg。
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| 关 键 词： | 鸡肉 泰妙菌素 残留 高效液相色谱-串联质谱法 |
Determination of Tiamulin Residues in Chicken Meat by HPLC-MS/MS |
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| GENG Shiwei
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ZHU Zhiqian
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QU Bin
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JIANG Tianmei
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LU Guiping.Determination of Tiamulin Residues in Chicken Meat by HPLC-MS/MS[J].China Poultry,2012,34(11):28-31. |
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| Authors: | GENG Shiwei ZHU Zhiqian QU Bin JIANG Tianmei LU Guiping |
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| Institution: | ( Jiangsu Provincial Test Center of Animal By-product Quality , Nanjing , Jiangsu 210036 ) |
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| Abstract: | A high performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/MS ) method was developed to determine tiamulin residues in chicken meat by solid phase extraction ( SPE ) . The sample was extracted by phosphate buffer ( pH 8.0 ) -ethyl acetate mixture solvent. After removing fat by water-saturated n-hexane , the sample was cleaned up by SPE using an oasis MCX extraction catridge. Tiamulin was detected by HPLC-MS/MS with MRM mode. It showed a good linearty in the range of 0.5 to 50 μg/L. The limit of detection was 0.25 μg/kg and the limit of quantification was 0.5 μg/kg. |
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| Keywords: | chicken meat tiamulin residue HPLC-MS/MS |
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